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| Loeza-Kuk,Emiliano; Gutiérrez-Espinosa,María A.; Ochoa-Martínez,Daniel L.; Villegas-Monter,Ángel; Mora-Aguilera,Gustavo; Palacios-Torres,Elvia C.; Pérez-Molphe-Balch,Eugenio. |
| En el manejo de la tristeza de los cítricos se ha recurrido a patrones tolerantes, pero tienen menor plasticidad que C. aurantium y la protección cruzada es superada en ocasiones por variantes más severas. Una alternativa puede ser plantas transgénicas con resistencia derivada del patógeno (PDR por sus siglas en inglés). Para evaluar la PDR conferida con la transgénesis plantas de Citrus parodisiy C. aurantifolia, transformadas vía Agrobacterium tumefaciens y A. rhizogenes con el gen p25 del Citrus tristeza virus (CTV), fueron multiplicadas en C. aurantium. y C. volkameriana e inoculadas por injerto y transmisión con Aphis gossypii con aislamientos mexicanos de CTV para determinar su resistencia. El diseño experimental usado fue completamente al azar en un... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Resistencia derivada del patógeno; Cítricos transgénicos; PCR tiempo real; Cuantificación viral. |
| Ano: 2011 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952011000100006 |
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| Tovar-Pedraza,Juan M.; Mora-Aguilera,José A.; Nava-Díaz,Cristian; Téliz-Ortiz,Daniel; Valdovinos-Ponce,Guadalupe; Villegas-Monter,Ángel; Hernández-Morales,Javier. |
| Dieback of mamey sapote (Pouteria sapota) grafts is the most important disease during vegetative propagation in commercial nurseries. In 2008, Lasiodiplodia theobromae identified by cultural, morphological and molecular characterization was associated with this disease in 97 % of necrotic rootstocks and scions samples from a nursery in Guerrero, México. The fungus was inoculated onto the binding site of El Mexicano grafted plants. Dieback symptoms and gradual drying with cracking of the bark from the apex to the base of the scion were observed 30 d after inoculation. Abundant mycelia and pycnidia were found on inoculated scions and graft union areas, in the periderm, and on leaf scars. Transverse sections of inoculated scions tissues showed pycnidia.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dieback; Nursery; Vegetative propagation; Pouteria sapota; Lasiodiplodia theobromae. |
| Ano: 2012 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000200005 |
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| Loeza-Kuk,Emiliano; Ochoa-Martínez,Daniel L.; Mora-Aguilera,Gustavo; Rivas-Valencia,Patricia; Gutiérrez-Espinosa,M. Alejandra; Cintra de Jesús Junior,Waldir; Villegas-Monter,Ángel; Arno-Wulff,Nelson; Perez-Molphe-Balch,Eugenio. |
| Individuals of Toxoptera citricida (Kirkaldy) and Aphis spiraecola (Patch) collected in 16 commercial citrus orchards of the São Paulo and Minas Gerais States of Brazil were analyzed for virus presence. Orchards of Minas Gerais were selected because citrus trees exhibit symptoms of citrus sudden death (CSD) and in São Paulo due the presence of severe strains of Citrus tristeza virus (CTV), in order to detect genomic sequences of any of these strains that could be associated to CSD. Samples made up of 20 aphids from the localities of Comendador Gomes, Frutal, Prata and Uberlândia, in the State of Minas Gerais, a CSD associated virus (CSDaV) was often found through nested RT-PCR; in samples from São Paulo, none showed positive for the same primers. With the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aphids; Virus detection; Citrus tristeza virus; Citrus sudden death-associated virus. |
| Ano: 2008 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952008000600007 |
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