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| Wang,Chun-jie; Yu,Siriguleng; Ao-ri-ge-le,; Jia,De-gang; Yao,Hong-qiang; Zhao,Huai-ping; Lillehoj,H.S.; Si-mu-ji-de,; Postnikoff,Andrew CL; Xu,Si-ri-gu-leng. |
| Two-day-old specific pathogen-free (SPF) chickens were divided into two groups. Group I was inoculated orally with fowl adenovirus VIII (FAV-VIII). Group II served as a negative control. Chickens were investigated at various days post-inoculation (dpi) by flow cytometric analysis for changes in T lymphocyte subpopulations in immune system and blood. In the thymus, CD3+ T lymphocytes were increased at 25 dpi, with significant increases in the FAV infected noted at 1, 12, 20dpi (p<0.05). This was accompanied by a corresponding increase of CD4+ and CD8+ T lymphocytes. In the spleen, CD3+ and CD4+ T lymphocytes were increased significantly at 30 dpi (p<0.01) whereas CD8+ and TCR γ δ+ T lymphocytes were decreased at 1 (p<0.05), 30 dpi (p<0.01). An... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell mediated immune competency; Fowl adenovirus VIII; Immune system; Peripheral blood; T lymphocyte subpopulations. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400007 |
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| Huasai,Simujide; Chen,Aorigele; Wang,Chun-jie; Li,Yu; Tongrige,Bai. |
| Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: E. coli; Virulence gene; Multiplex PCR; Serogroup; Healthy dairy cow. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200013 |
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