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Effects of E. Coli Infection on the Expressions of TGF-β/Smads Signaling Pathway in Broiler Intestine Rev. Bras. Ciênc. Avic.
Juang,J; Yin,H; Zhang,C; Wang,J.
ABSTRACT This experiment aimed to investigate whether Escherichia coli (E. coli) infection could affect the TGF-β/smads signaling pathway in the jejunal tissue of chickens. One-day-old Cobb 500 broilers were randomly divided into 2 groups and treated with intraperitoneal E. coli or broth injection. Clinical signs of the birds were assessed every day. Spleen and bursa of Fabricius of the birds, post-infection (pi), were collected to evaluate immune organ index. Jejunal tissues were collected to ascertain the expression of TGF-βs, TβRs, and Smads. The results showed that the infected birds had significantly higher index of the spleen (24hrs and 48hrs pi) compared with birds in the control group (p<0.05). The relative gene expression of TGF-β4 increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TGF-β/Smads; Intestinal lesion; E. coli; Chicken.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100311
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Molecular Characterization of Goose Phosphoenolpyruvate Carboxylase Kinase 1 (Pepck) Gene and Its Potential Role in Hepatic Steatosis Induced by Overfeeding Rev. Bras. Ciênc. Avic.
Tang,B; Yang,C; Hu,S; Sun,W; Pan,Z; Li,L; Wang,J.
ABSTRACT Over-accumulation of triglycerides (TGs) in goose hepatocytes leads to the formation of fatty acid liver. Phosphoenolpyruvate carboxylase kinase 1 (PEPCK) is regarded as the rate-limiting enzyme for gluconeogenesis, and there is evidence that PEPCK is involved in regulating hepatic glucolipid metabolism. Hence, we proposed that PEPCK may have a role in goose hepatic steatosis. To test our hypothesis, the present study was conducted to firstly determine the sequence characteristics of goose PEPCK and then to explore its role in overfeeding-induced fatty liver. Our results showed that goose PEPCK encodes a 622-amino-acids protein that contains highly conserved oxaloacetate-binding domain, kinase-1 and kinase-2 motifs. PEPCK had higher mRNA levels in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goose; PEPCK; Sequence characteristics; Hepatic steatosis; Primary hepatocytes.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400302
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The Gene Ontology Differs in Bursa of Fabricius Between Two Breeds of Ducks Post Hatching by Enriching the Differentially Expressed Genes Rev. Bras. Ciênc. Avic.
Liu,H; Liu,J; Zhang,T; Li,L; Wang,J; Han,C; He,H.
ABSTRACT The bursa of Fabricius (BF) is the central humoral immune organ unique to birds. The present study investigated the possible difference on a molecular level between two duck breeds. The digital gene expression profiling (DGE) technology was used to enrich the differentially expressed genes (DEGs) in BF between the Jianchang and Nonghua-P strains of ducks. DGE data identified 195 DEGs in the bursa. Gene Ontology (GO) analysis suggested that DEGs were mainly enriched in the metabolic pathways and ribosome components. Pathways analysis identified the spliceosome, RNA transport, RNA degradation process, Jak-STAT signaling pathway, TNF signaling pathway and B cell receptor signaling pathway. The results indicated that the main difference in the BF...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bursa of Fabricius; Gene ontology; Duck; Digital gene expression profiling..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100169
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Suppresses of Astragalus Polysaccharide on E. coli-Induced Injured Intestinal Microvascular through TLR4-NF-κB Signal Pathways in Chickens Rev. Bras. Ciênc. Avic.
Su,L; Wang,J; Huang,J; Zhao,Y; Jiang,H; Li,H.
ABSTRACT To investigate the hypothesis that APS can attenuate E. coli-induced microvascular dysfunction in chicken intestine, 60 0-day old male chickens were divided into three groups with 5 replications of 4 chicks. Chicken in the APS group were treated with 15 mg APS daily while the others were given 0 mg APS for 6 days. Then all 7-day old chicken were injected intraperitoneally by E. coli, except for the chicken in the control group. After 4 h, all chicken’s intestinal samples were collected to detect gene expressions involved in inflammatory factors and adhesion molecules. Results showed that APS attenuated the signs of edema and hemorrhage in 7-day old chicken intestinal mucosa which were induced by E. coli injection. Consistently, APS significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astragalus polysaccharide; Escherichia coli; Intercellular adhesion molecule -1; Nuclear factor - κB; Vascular cell adhesion molecule -1.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300302
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