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| Ding,Fang; Yuan,Xin; Li,Qingqing; Sun,Wenqiang; Gan,Chao; He,Hua; Song,Chenling; Wang,Jiwen. |
| Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation. Results We have isolated a 7654 bp fragment from Peking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05).... |
| Tipo: Journal article |
Palavras-chave: Expression pattern; FAS; Oleic acid; Peking duck. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600001 |
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| Li,Xinxin; Wang,Jiwen; Liu,Hehe; Wang,Haohan; Sun,Lingli; Yang,Chao; Li,Liang; Zheng,Yi. |
| Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN). Results The duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in... |
| Tipo: Journal article |
Palavras-chave: Follistatin; Muscle hypertrophy; Overexpression; Transfection efficiency. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500006 |
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| Liu,Hehe; Wang,Jiwen; Si,Jianmin; Jia,Jing; Li,Liang; Han,Chunchun; Huang,Kailiang; He,Hua; Xu,Feng. |
| The role of myogenic enhancer transcription factor 2a (MEF2A) in avian muscle during fetal development is unknown. In this work, we cloned the duck MEF2A cDNA sequence (GenBank accession no. HM460752) and examined its developmental expression profiles in cardiac muscle, non-vascular smooth muscle and skeletal muscle. Duck MEF2A cDNA comprised 1479 bp encoding 492 amino acid residues. In silico analysis showed that MEF2A contained MADS (MCM1, AGAMOUS, DEFICIENS and SRF -serum response factor), MEF2 and mitogen-activated protein kinase (MAPK) transcription domains with high homology to related proteins in other species. Modified sites in these domains were conserved among species and several variants were found. Quantitative PCR showed that MEF2A was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duck MEF2A; Expression profile; In silico analysis; Molecular cloning; Muscle tissues. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100026 |
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| Wang,Haohan; Li,Xinxin; Liu,Hehe; Sun,Lingli; Zhang,Rongping; Li,Liang; Wangding,Mincheng; Wang,Jiwen. |
| Abstract As a critical transcription factor, Six1 plays an important role in the regulation of myogenesis and muscle development. However, little is known about its regulatory mechanism associated with muscular protein synthesis. The objective of this study was to investigate the effects of overexpression ofSix1 on the expression of key protein metabolism-related genes in duck myoblasts. Through an experimental model where duck myoblasts were transfected with a pEGFP-duSix1 construct, we found that overexpression of duckSix1 could enhance cell proliferation activity and increase mRNA expression levels of key genes involved in the PI3K/Akt/mTOR signaling pathway, while the expression of FOXO1, MuRF1and MAFbx was not significantly altered, indicating... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duck; Six1; Protein metabolism signaling; MTOR; PEGFP-duSix1. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100151 |
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