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Chen,Yong-Hao; Li,Jun; Liu,Li; Liu,Hong-Zhi; Wang,Qiang. |
A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus equisimilis mutant; Hyaluronic acid; Optimization; Culture medium; Culture conditions. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400040 |
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Lv,Dan; Pan,Li-hong; Zhang,Ren; Yang,Jie; Chen,Hao; Wen,Yanzhang; Huang,Mi; Ma,Xinhua; Wang,Qiang; Yang,Xinzhou. |
Hepatocellular carcinoma is one of the most prevalent malignancies and a leading cause of cancer-related mortality worldwide. However, the therapies to prevent hepatocellular carcinoma are still limited and the emergence of drug resistance leads to the development of new anti-cancer drugs and combinational chemotherapy regimens. Our study was aimed to explore the anticancer effects of the essential oil extract (EEEO) from Euphorbia esula which has been widely used in traditional Chinese folk medicine and possessed potential cytotoxic effects in several human tumor cells. However, the mechanisms of EEEO-induced anti-proliferation and apoptosis have not been completely elucidated. In this study, EEEO was prepared by hydro-distillation and the main chemical... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Euphorbia esula; Hepatocellular carcinoma; Apoptosis; Reactive oxygen species; Mitochondrial pathway. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100506 |
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