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Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity Biol. Res.
Yan,Yizhong; Li,Jianjun; Zhang,Yiya; Peng,Xiaozhen; Guo,Tianyao; Wang,Jirong; Hu,Weijun; Duan,Zhigui; Wang,Xianchun.
BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block...
Tipo: Journal article Palavras-chave: Black widow spider (L. tredecimguttatus); Protein composition; Egg; Molecular mechanism; Property analysis; Toxicity.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017
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Detection and identification of huwentoxin-IV interacting proteins by biotin-avidin chemistry combined with mass spectrometry J. Venom. Anim. Toxins incl. Trop. Dis.
Yu,Hai; Liu,Hui; Yan,Yizhong; Duan,Zhigui; Wang,Xianchun.
Background : Numerous spider toxins are of interest as tools for neurophysiological research or as lead molecules for the development of pharmaceuticals and insecticides. Direct detection and identification of the interacting proteins of a spider toxin are helpful for its action-mechanism analysis and practical application. The present study employed a combinative strategy for the analysis of interacting proteins of huwentoxin-IV (HWTX-IV), a peptidic neurotoxin from the venom of the spiderSelenocosmia huwena.Results : HWTX-IV was first lightly labeled with biotin under the optimized mild experimental conditions and the toxin labeled with a single biotin group (monobiotinylated HWTX-IV) was demonstrated by electrophysiological experiments to retain its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotin labeling; Huwentoxin-IV; Far-western blotting; Interacting protein; Mass spectrometry.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200328
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