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| Zhang,P.; Wang,C.T.; Yan,F.; Gou,L.; Tong,A.P.; Cai,F.; Li,Q.; Deng,H.X.; Wei,Y.Q.. |
| Mouse PNAS-4 (mPNAS-4) has 96% identity with human PNAS-4 (hPNAS-4) in primary sequence and has been reported to be involved in the apoptotic response to DNA damage. However, there have been no studies reported of the biological functions of mPNAS-4. In studies conducted by our group (unpublished data), it was interesting to note that overexpression of mPNAS-4 promoted apoptotic death in Lewis lung carcinoma cells (LL2) and colon carcinoma cells (CT26) of mice both in vitro and in vivo. In our studies, mPNAS-4 was cloned into the pGEX-6P-1 vector with GST tag at N-terminal in Escherichia coli strain BL21(DE3). The soluble and insoluble expression of recombinant protein mPNAS-4 (rmPNAS-4) was temperature-dependent. The majority of rmPNAS-4 was insoluble at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anti-mPNAS-4; Polyclonal antibody; Apoptosis-related protein family; One-step affinity purification. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600012 |
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| Wan,Y.; Tang,M.H.; Chen,X.C.; Chen,L.J.; Wei,Y.Q.; Wang,Y.S.. |
| Immune response plays an important role in the development of hepatic fibrosis. In the present study, we investigated the effects of quercetin on hepatitis and hepatic fibrosis induced by immunological mechanism. In the acute hepatitis model, quercetin (2.5 mg/kg) was injected iv into mice 30 min after concanavalin A (Con A) challenge. Mice were sacrificed 4 or 24 h after Con A injection, and aminotransferase tests and histopathological sections were performed. Treatment with quercetin significantly decreased the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Consistent with this observation, treatment with quercetin markedly attenuated the pathologic changes in the liver. A hepatic fibrosis model was also generated in mice... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quercetin; Concanavalin A; Acute hepatitis; Hepatic fibrosis; Liposome. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800655 |
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