| Ten healthy 2 to 4-week-old Holstein calves were randomly allotted into control and infected groups. Control calves (n=5) were inoculated intrabronchially with 5ml of Dulbecco's phosphate-buffered saline solution (DPBSS). Infected calves (n=5) were inoculated intrabronchially with 5x10(9) log-phase Mannheimia haemolytica organisms suspended in 5ml of DPBSS. Blood samples were obtained 15 minutes before and one, two, four and six hours after inoculation. Serum protein concentrations were determined by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Serum concentrations of proteins with molecular weights of 125,000 D (ceruloplasmin), 60,000 D (a 1-antitrypsin), 45,000 D (haptoglobin), and 40,000 D (acid glycoprotein) were significantly... |
