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Bovine semen thermoresistance tests and their correlation with pregnancy rates after fixed-time artificial insemination Arq. Bras. Med. Vet. Zootec.
Talini,R.; Kozicki,L.E.; Gaievski,F.R.; Polo,G.; Lima,L.G.F.; Santiago,J.; Segui,M.S.; Weiss,R.R.; Galan,T.G.B..
ABSTRACT The objective of the study was to determine whether or not there is a correlation between thermoresistance tests (TT) after semen thawing and pregnancy rate (PR) after fixed-time artificial insemination (FTAI). Four different TT were performed on ten samples used for AI; a rapid test (RTT) (30min / 46°C) and three slow tests (STT): STT1 (60min/38°C), STT2 (180min/38°C), and STT3 (300min/38°C). Two hundred and fifteen multiparous crossbred cows were submitted to FTAI under the following protocol: on day zero (d0) the animals received a P4 device +EB; on d7 PGF2α; on d8 P4 was removed and eCG+EC were administered; IATF was performed on d10. Three gestational diagnoses (G D) were performed on d40, d70 and d120. The mean sperm motility (%) in RTT and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sperm motility; Casa; Pregnancy rate; Beef cows; Semen thermoresistance test.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000602085
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Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen Arq. Bras. Med. Vet. Zootec.
Bergstein,T.G.; Bicudo,L.C.; Rodello,L.; Weiss,R.R.; Bicudo,S.D..
ABSTRACT Frozen and thawed ovine semen undergo morphological and functional changes that prevent or decrease the efficiency of fertilization. Sperm selection methods seek to improve the quality and viability of the fertilizing materials. Four sperm selection methods were employed, using two silica colloidal solutions coated with silane (silica colloidal-silane) or by polyvinylpyrrolidone (silica colloidal-PVP), and varying the volume of colloidal solution. Sperm kinematic and sperm recovery were evaluated by means of CASA. The protocols using silica colloidal-silane showed higher total motility (TM), progressive motility (PM) and percentage of rapid sperm (%RAP) compared to the methods employing silica colloidal-PVP and to the samples prior to sperm...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovine; Semen; Silane; Selection; Colloidal silica.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601539
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Viability of ovine spermatozoa collected from epididymides stored at 18°-25°C for 48 hours post mortem Arq. Bras. Med. Vet. Zootec.
Bergstein-Galan,T.G.; Weiss,R.R.; Barbosa,T.S.R.; Kozicki,L.E.; Bicudo,S.D..
ABSTRACT The objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25°C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0±1.9%) compared to AV (86.4±1.0%). PM decreased (P<0.05) from 12 hours after death (31.3±4.0%) compared to AV group (73.2±1.4%). The percentage of viable cells in HOST decreased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Epididymis; Room temperature.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401023
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