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Template preparation for rapid PCR in Colletotrichum lindemuthianum 58
Roca,M. Gabriela; Davide,Lisete C.; Wheals,Alan E..
Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracnose; Hygromycin resistance; Internal transcribed spacer; Rapid screening; PCR methodology.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003
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Isolation and identification of yeasts and filamentous fungi from yoghurts in Brazil 58
Moreira,Silvia Regina; Schwan,Rosane Freitas; Carvalho,Eliana Pinheiro de; Wheals,Alan E..
Seventy-two cartons of yoghurt were sampled three times at monthly intervals from four different local manufacturers. Total counts were close to 6 x 10(7) cells g-1 of yoghurt. Yeast counts varied from 1 to 2,700 g-1. There was no evidence of systematic contamination at source but this longitudinal study revealed that ad hoc contamination and improper storage led to the higher yeast counts. Contamination was generally higher in the hotter months but was lower overall than reported from other countries. A total of 577 yeast isolates were identified belonging to ten species. The most abundant yeasts were, in order, Debaryomyces hansenii, Saccharomyces cerevisiae, Mrakia frigida, Hansenula spp., Candida parapsilosis, Debaryomyces castellii and Candida...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeasts; Filamentous fungi; Yoghurts; Deterioration.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200009
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