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Data Mining of the Coffee Rust Genome Nature Precedings
David Octavio Botero-Rozo; William Giraldo; Alvaro Gaitan; Marco Cristancho; Diego M. Riaño-Pachon; Silvia Restrepo.
The genomes of nine isolates of _Hemileia vastatrix_, the causal agent of coffee leaf rust were sequenced by Illumina and 454. Quality control, cleaning and _de novo_ assemblies of data were performed. Since isolates were obtained from the field and it is not possible to produce axenic cultures of _H. vastatrix_, MEGAN software was used to evaluate contamination levels and to select contigs with fungal similarities. Mitochondrial contigs were identified and annotated by comparing this assembly against the _Puccinia_ genome. Furthermore, two transcriptomes from isolates of _H. vastatrix_ were assembled to complement the genomic data.
Tipo: Poster Palavras-chave: Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/7034/version/1
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