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	Provedor de dados Electron. J. Biotechnol. (2) Autor Illanes,Andrés (2) Wilson,Lorena (2) González,José Miguel (1) Gómez,José Manuel (1) Romero,Oscar (1) Valencia,Pedro (1) Mais... Palavra-chave CLEAs (1) Diffusional restrictions (1) Enzyme immobilization (1) Enzyme kinetics (1) Enzyme reactivation (1) Glyoxyl agarose (1) Immobilized enzyme (1) Inactivation (1) Organic cosolvents (1) Penicillin G acylase (1) Penicillin hydrolysis (1) Unfolding - refolding (1) Mais... Tipo do documento Journal article (2) Ano 2013 (1) 2010 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts Electron. J. Biotechnol. Wilson,Lorena; Illanes,Andrés; Romero,Oscar. Enzymes are labile catalysts with reduced half-life time that can be however improved by immobilization and, furthermore, already inactivated catalyst can be recovered totally or partially, therefore allowing the large scale application of enzymes as process catalysts. In recent years a few studies about reactivation of enzyme catalysts have been published as a strategy to prolong the catalyst lifetime. Reported results are very good, making this strategy an interesting tool to be applied to industrial process. These studies have been focused in the evaluation of different variables that may have a positive impact both in the rate and level of activity recovery, being then critical variables for conducting the reactivation process at productive scale. The... Tipo: Journal article Palavras-chave: CLEAs; Enzyme immobilization; Enzyme reactivation; Glyoxyl agarose; Inactivation; Organic cosolvents; Unfolding - refolding. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300015 Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading Electron. J. Biotechnol. Illanes,Andrés; González,José Miguel; Gómez,José Manuel; Valencia,Pedro; Wilson,Lorena. Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized penicillin G acylase biocatalysts of average particle size of 5·10-5m and 10·10-4m at protein loadings from 15 to 130 mg/g gel. Internal diffusional restrictions were evaluated for such biocatalysts: Thiele modulus varied from 1.17 for the small particles at the lower protein load to 5.84 for the large particles at the higher protein load. Effectiveness factors at different bulk substrate concentrations were determined for all biocatalysts, values ranging from... Tipo: Journal article Palavras-chave: Diffusional restrictions; Enzyme kinetics; Immobilized enzyme; Penicillin G acylase; Penicillin hydrolysis. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100002 Registros recuperados: 2 Primeira ... 1 ... Última

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