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| Zhou,X.Q.; Huang,S.Y.; Zhang,D.S.; Zhang,S.Z.; Li,W.G.; Chen,Z.W.; Wu,H.W.. |
| Reversion-inducing cysteine-rich protein with kazal motifs (RECK), a novel tumor suppressor gene that negatively regulates matrix metalloproteinases (MMPs), is expressed in various normal human tissues but downregulated in several types of human tumors. The molecular mechanism for this downregulation and its biological significance in salivary adenoid cystic carcinoma (SACC) are unclear. In the present study, we investigated the effects of a DNA methyltransferase (DNMT) inhibitor, 5-aza-2′deoxycytidine (5-aza-dC), on the methylation status of the RECK gene and tumor invasion in SACC cell lines. Methylation-specific PCR (MSP), Western blot analysis, and quantitative real-time PCR were used to investigate the methylation status of the RECK gene and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RECK; Salivary adenoid cystic carcinoma; 5-aza-2′deoxycytidine; Methylation; Transwell assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300254 |
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