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Renal Dose Dopamine Mediates the Level of Aquaporin-2 Water Channel (Aqp2) in Broiler Chickens Rev. Bras. Ciênc. Avic.
Chang,Z; Zhang,H; Wu,X; Nabi,F; Rehman,MU; Yuan,X; Mehmood,K; Zhou,D.
ABSTRACT Aquaporin 2 (AQP2) is a small protein located in the collecting tubules of kidneys; it plays an important role in the concentration and production of urine. The aim of this study was to determine the expression level of the AQP2 gene in the kidney of broiler chickens after the administration of renal­dose dopamine. Broiler chickens (25 days-old) were randomly divided into two groups (n=20 per group): intravenous administration of saline solution (control group) or renal-dose dopamine (dopamine group). The expression and localization of the AQP2 gene were evaluated by real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC), respectively. The protein level of AQP2 was analyzed by western blot analysis. The dopamine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AQP2; Renal dose dopamine; Kidney; Broiler chickens.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300387
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Epigallocatechin Gallate (EGCG) Inhibited the Alv-J-Induced Apoptosis in Df-1 Cells by Inactivation of Nuclear Factor κb Pathway Rev. Bras. Ciênc. Avic.
Zhang,Y; Yang,H; Wu,X; Deng,M; Li,Z; Xu,Z.
ABSTRACT Avian leukosis virus subgroup J (ALV-J), a member of the retroviridae family, can infect both broilers and layers and induce a spectrum of different neoplasms, resulting in serious economic losses in poultry production. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, has demonstrated remarkable anti-inflammatory and cancer chemopreventive effects in many animal tumor bioassays, cell culture systems and epidemiological studies. To assess the antiviral effects of EGCG on ALV-J-induced cell apoptosis in vitro, DF-1 cells were treated with different EGCG concentrations (0, 5, 10, 20 and 40 µg/mL), and their antiviral effects were examined at different time points (0, 24, 48, 72 and 96 h) using a variety of assays. EGCG...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ALV-J; EGCG; NF-kB; DF-1 cell; Apoptosis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100323
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