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Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09 Anais da ABC (AABC)
MA,YUPING; SUN,SIWEN; HAO,HUI; XU,CHUNPING.
ABSTRACT A soil isolate, Penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-PG). By optimizing growth conditions, P. janthinellum sw09 produced high amount of exo-PG (16.54 units/mL). The crude enzyme was purified by gel filtration chromatography and two exo-PG activity peaks (designated as PGI and PGII) were revealed. On SDS-PAGE analysis, purified PGII using DEAE-Sepharose FF column, was found to be a single band with a molecular mass of 66.2 kDa. The purified PGII exhibited maximal activity at the temperature of 45 oC and pH 5.0. The stability profiles show that PGII is more stable in the pH range of 4.0-8.0 and below 60 oC. The Km and Vmax...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillium janthinellum; Polygalacturonase; Purification; Characterization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200479
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Effect of carbon source on production, characterization and bioactivity of exopolysaccharide produced by Phellinus vaninii Ljup Anais da ABC (AABC)
XU,CHUNPING; YU,JINWEI; ZHAO,SHANSHAN; WU,SHUNGSHUNG; HE,PEIXIN; JIA,XUEWEI; LIU,YANQI; MAO,DUOBIN.
ABSTRACT The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum EPS yield was achieved when sucrose was employed. The predominant carbohydrate compositions in EPSs identified were gluconic acid, glucose, mannose and galactose acid. Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs. EPSs molecule exist as nearly globular shape form in aqueous solution. The variation also affects antioxidant activities by investigated by using hydroxyl and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phellinus vaninii Ljup; Exopolysaccharide; Carbon source; Antioxidant activity; Composition.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502033
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Simultaneous Determination of Furan and Vinyl Acetate in Vapor Phase of Mainstream Cigarette Smoke by GC-MS Anais da ABC (AABC)
XU,AIFEI; FAN,ZHONG; CHEN,ZHIYAN; ZHOU,YUN; LIU,SHAOHUA; HUANG,SHIJIE; XU,XUEQUIN; LONG,CONGYUN; WANG,WEIGANG; ZHU,JING; LI,XIAOLAN; XU,CHUNPING.
ABSTRACT A simple and sensitive method for simultaneous determination of furan and vinyl acetate (VA) in vapor phase of mainstream cigarette smoke with cold trap and gas chromatography-mass spectrometry (GC-MS) was developed. A Cambridge filter pad (CFP) was placed in front of the impingers of smoking machine to remove the particle phase from cigarette smoke. Furan and VA in vapor phase of mainstream cigarette smoke were collected in two impingers connected in series by filled with methanol at -78°C. The solutions were added with deuterium-labeled furan-d4 and VA-d6 as internal standards and analyzed by GC-MS. The results showed that the calibration curves for furan and VA were linear (r2 > 0.9995) over the studied concentration range. The intra- and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vapor phase; Mainstream cigarette smoke; Furan; Vinyl acetate; Cold trap; Gas chromatography-mass spectrometry.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200383
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