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| Gong,Zhen; Liu,Jianyun; Xie,Xin; Xu,Xiaoyuan; Wu,Ping; Li,Huimin; Wang,Yaqin; Li,Weidong; Xiong,Jianjun. |
| Abstract The ubiquitin-specific protease 22 (USP22) is an oncogene and its expression is upregulated in many types of cancer. In the nucleus, USP22 functions as one subunit of the SAGA to regulate gene transcription. However, the genome-wide USP22 binding sites and its direct target genes are yet clear. In this study, we characterized the potential genomic binding sites of UPS22 and GCN5 by ChIP-seq using specific antibodies in HeLa cells. There were 408 overlapping putative target genes bound by both USP22 and GCN5. Motif analysis showed that the sequences bound by USP22 and GCN5 shared two common motifs. Gene ontology (GO) and pathway analysis indicated that the genes targeted by USP22 and GCN5 were involved in different physiological processes and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: USP22; Target genes; ChIP-seq; Knockdown; RNA-seq. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300488 |
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| Hu,Jun; Xie,Xin; Li,Yuan; Wang,Shuang; Feng,Qing; Wang,Xin; Liang,Daoyan. |
| Prompt and accurate detection of rejection prior to pathological changes after organ transplantation is vital for monitoring rejections. Although biopsy remains the current gold standard for rejection diagnosis, it is an invasive method and cannot be repeated daily. Thus, noninvasive monitoring methods are needed. In this study, by introducing an IL-2 neutralizing monoclonal antibody (IL-2 N-mAb) and immunosuppressants into the culture with the presence of specific stimulators and activated lymphocytes, an activated lymphocyte-specific assay (ALSA) system was established to detect the specific activated lymphocytes. This assay demonstrated that the suppression in the ALSA test was closely related to the existence of specific activated lymphocytes. The ALSA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heart transplantation rejection; Activated lymphocytes; Noninvasive method; Primed lymphocyte typing; Interleukin-2 neutralization monoclonal antibody; ALSA test. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200012 |
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