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	Provedor de dados BJM (2) Autor Bisht,Deepali (2) Darmwal,Nandan Singh (2) Yadav,Santosh Kumar (2) Palavra-chave Alkaline lipase (2) Box-Behnken design (1) Detergent stability (1) Mutant (1) Organic-solvent (1) P-nitrophenyl palmitate Pseudomonas (1) Pseudomonas aeruginosa (1) Response surface methodology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última An oxidant and organic solvent tolerant alkaline lipase by P. aeruginosa mutant: downstream processing and biochemical characterization BJM Bisht,Deepali; Yadav,Santosh Kumar; Darmwal,Nandan Singh. An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lipase has an apparent molecular mass of 67 kDa. The optimum temperature and pH for the purified lipase were 45°C and 8.0, respectively. The enzyme showed considerable stability in pH range of 7.0-11.0 and temperature range 35-55 °C. The metal ions Ca2+, Mg2+ and Na+ tend to increase the enzyme activity, whereas, Fe2+ and Mn2+ ions resulted in discreet decrease in the activity. Divalent cations Ca+2 and Mg+2 seemed to protect the enzyme against thermal denaturation... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline lipase; Detergent stability; Mutant; Organic-solvent; P-nitrophenyl palmitate Pseudomonas. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400040 Computation of interactive effects and optimization of process parameters for alkaline lipase production by mutant strain of Pseudomonas aeruginosa using response surface methodology BJM Bisht,Deepali; Yadav,Santosh Kumar; Darmwal,Nandan Singh. Alkaline lipase production by mutant strain of Pseudomonas aeruginosa MTCC 10,055 was optimized in shake flask batch fermentation using response surface methodology. An empirical model was developed through Box-Behnken experimental design to describe the relationship among tested variables (pH, temperature, castor oil, starch and triton-X-100). The second-order quadratic model determined the optimum conditions as castor oil, 1.77 mL.L-1; starch, 15.0 g.L-1; triton-X-100, 0.93 mL.L-1; incubation temperature, 34.12 ºC and pH 8.1 resulting into maximum alkaline lipase production (3142.57 U.mL-1). The quadratic model was in satisfactory adjustment with the experimental data as evidenced by a high coefficient of determination (R²) value (0.9987). The RSM... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline lipase; Box-Behnken design; Pseudomonas aeruginosa; Response surface methodology. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100035 Registros recuperados: 2 Primeira ... 1 ... Última

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