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	Provedor de dados BJM (1) Autor Asghar Karkhane,Ali (1) Farrokh,Parisa (1) Yakhchali,Bagher (1) Palavra-chave Alkaline (1) Enterobacter (1) Organic solvent (1) Thermostable lipase (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2014 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Cloning and characterization of newly isolated lipase from Enterobacter sp. Bn12 BJM Farrokh,Parisa; Yakhchali,Bagher; Asghar Karkhane,Ali. A mesophilic Enterobacter sp. Bn12 producing an alkaline thermostable lipase was isolated from soil in Tehran, Iran. The lipase gene (ELBn12) was identified from a genomic library. Sequence analysis of the DNA fragment revealed an open reading frame of 879 bp encoding a lipase with a molecular mass of 31.3 kDa. The deduced amino acid sequence showed 96% identity with a lipase of Enterobacter sp. Ag1 and the identity of their DNA sequences was 88.9%. ELBn12 belongs to the lipase subfamily I.1 and its catalytic triad consists of Ser82, Asp237 and His259. The lipase was expressed in Escherichia coli (BL21) pLysS and partially purified by anion exchange chromatography. The maximum activity of ELBn12 was obtained at temperature of 60 °C and pH 8.0 towards... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacter; Thermostable lipase; Alkaline; Organic solvent. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200042 Registros recuperados: 1 Primeira ... 1 ... Última

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