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| Poersch-Bortolon,Liane Balvedi; Scagliusi,Sandra Maria Mansur; Yamazaki-Lau,Elene; Bodanese-Zanettini,Maria Helena. |
| Abstract The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4ºC for 21 days; and 2-hydroxynicotinic acid, at 32ºC for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum; Albinism; Androgenesis; Doubled haploid; Isolated microspore culture; Recalcitrance. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101839 |
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| Mar,Talita Bernardon; Lau,Douglas; Schons,Jurema; Yamazaki-Lau,Elene; Nhani Jr.,Antônio. |
| Yellow dwarf disease, one of the most important diseases of cereal crops worldwide, is caused by virus species belonging to the Luteoviridae family. Forty-two virus isolates obtained from oat (Avena sativa L.), wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), corn (Zea mays L.), and ryegrass (Lolium multiflorum Lam.) collected between 2007 and 2008 from winter cereal crop regions in southern Brazil were screened by polymerase chain reaction (PCR) with primers designed on ORF 3 (coat protein - CP) for the presence of Barley yellow dwarf virus and Cereal yellow dwarf virus (B/CYDV). PCR products of expected size (~357 bp) for subgroup II and (~831 bp) for subgroup I were obtained for three and 39 samples, respectively. These products were cloned... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Luteoviridae; Poaceae; Small grains; Wheat; Oat. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000600008 |
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