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| Zhao,Ting; Ye,Zhixu; Wang,Binlin; Cui,Yuxia; Nie,Yingjie; Yang,Bin; Chen,Kun; Zhang,Hua; Hu,Fangfang; Yu,Fuxun. |
| ABSTRACT To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3' terminal of glycoprotein gene (HVR2) sequence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human respiratory syncytial virus; Virus isolation; RT-PCR; HVR2; Phylogenetic analysis; Molecular epidemiology. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600427 |
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| Chen,Lin; Huang,Zhuochun; Liao,Yun; Yang,Bin; Zhang,Junlong. |
| Tumor necrosis factor-alpha (TNF-α) plays an important role in autoimmune diseases. Previous studies have investigated the association of TNF-α-238G/A (rs361525) and -308G/A (rs1800629) polymorphisms with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). However, no agreed conclusion had been made. Therefore, this meta-analysis was conducted to assess the associations of TNF-α-238G/A and -308G/A polymorphisms with RA and SLE risk. A systematic search was conducted in commonly used databases. Meta-analysis was performed by STATA12.0. A total of 43 studies were included. In the overall population, the TNF-α-238A allele was observed to be a protective factor for RA (A vs G: OR=0.75, 95%CI=0.57–0.99, P=0.040) and the TNF-α-308A allele was found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rheumatoid arthritis; Single-nucleotide polymorphism; Systemic lupus erythematosus; Tumor necrosis factor-alpha; Meta-analysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300607 |
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