|
|
|
|
|
Zhang,Tianxiang; Peng,Dong; Qi,Lei; Li,Weixuan; Fan,Mengyuan; Shen,Jiachen; Yang,Liangliang; Wang,Yihua; Wang,Wenxia; Hu,Xiaolong; Cai,Ruibo; Zhou,Ran; Wei,Yuting; Zhou,Juntong; Yang,Shuang; Hu,Defu; Liu,Shuqiang. |
Abstract Background The muskrat is a seasonal breeder. Males secrete musk to attract females during the breeding season. The testosterone binding to the androgen receptor (AR) in musk glands of muskrat may play an important role conducting the musk secretion process. Methods The musk gland, testis and blood samples of musk rats are collected in both breeding and non-breeding seasons. Some part of the samples are kept in liquid nitrogen for transcriptome analysis and Western blotting test. Some part of the samples are kept in 70% alcohol for histology experiment, blood samples are kept at −20 °C for the serum testosterone measurement experiment. Results This study demonstrates that the quantity of secreted musk, the volume of the musk... |
Tipo: Journal article |
Palavras-chave: Muskrat; Musk gland; Testis; AR; Seasonal development. |
Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100201 |
| |
|
|
Tian,Meili; Yang,Shuang; Yan,Xinpeng. |
Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay, and Annexin V-FITC/PI double staining method were carried out to determine the changes of HCT116 cell growth and migration. mRNA and protein expressions were determined by quantitative real-time PCR and western blot, respectively. Moreover, small interfering RNA (siRNA) plasmid was used to examine the role of p53 in icariin-induced apoptosis in HCT116 cells. Icariin significantly suppressed colon... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colon carcinoma; Icariin; Mechanism; P53; DNA damage; Caspases. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000605 |
| |
|
|
Yang,Shuang; Zheng,Mingzhu; Cao,Yong; Dong,Yanjiao; Yaqoob,Sanabil; Liu,Jingsheng. |
Abstract Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the ability of biotransformation zein. The optimized fermentation conditions were as follows: inoculum concentration of 19%, volume of liquor of 130 mL/500 mL and pH of 4.7. Under this condition, the degree of hydrolysis (DH) was 27.31%. The zein hydrolysates from fungi fermentation maintained a high thermal stability. Compared to the original zein, the zein hydrolysates were found to have high solubility, which most likely results in improved foaming and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zein; Degree of hydrolysis; Cordyceps militaris 202; Functionality. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300621 |
| |
|
|
|