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	Provedor de dados BABT (1) Autor Hu,Tingzhang (1) Tan,Lili (1) Wu,Yingmei (1) Yang,Junnian (1) Yang,Yongwei (1) Palavra-chave GST activity (1) Glutathione S-transferase (1) In silico (1) Oryza sativa L. (1) Recombinant expression (1) Semi-quantitative RT-PCR (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2014 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Molecular characteristics, recombinant expression and activity detection of OsGSTL1 from rice BABT Hu,Tingzhang; Yang,Yongwei; Tan,Lili; Yang,Junnian; Wu,Yingmei. The mRNA of OsGSTL1 was detected in the roots and leaves of rice plants at seedling and tillering stages, and their roots, leaves and panicles at the heading stage. The full-length open reading frame of OsGSTL1 cDNA was 732 bp and encoded a putative polypeptide of 243 amino acids with a calculated molecular mass of 27.30 kDa and a theoretical pI of 5.50. The protein sequences of OsGSTL1 exhibited typical feature of the lambda class GST, which contained the conserved domain "GST_C_Lambda" in C-terminal alpha helical domain and a highly conserved Cys42 in active center. In silico predictions showed that the OsGSTL1 protein was strongly hydrophilic. The phylogenetic analysis revealed OsGSTL1 belonged to monocots subgroup and was closer to IN2-1 of Z. may. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione S-transferase; GST activity; In silico; Oryza sativa L.; Recombinant expression; Semi-quantitative RT-PCR. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100011 Registros recuperados: 1 Primeira ... 1 ... Última

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