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Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions BJM
Silva,Fábio Sérgio B.; Yano-Melo,Adriana M.; Brandão,Joana Angélica C.; Maia,Leonor C..
The production of inoculum is one of the hindrances in the large scale application of arbuscular mycorrhizal fungi (AMF). The objective of this work was to evaluate the effect of nutrient solutions with or without Tris-HCl buffer, on sporulation of AMF. The experiment was carried out in a greenhouse, using a substrate with sand and vermiculite (1:1 v/v). Fifty spores of Gigaspora margarita, Scutellospora heterogama, and Glomus etunicatum were inoculated in Sorghum vulgare (sorghum) or Panicum miliaceum (fodder millet). The substrate received the following nutrient solutions: Hoagland with 3 muM P (S1); Long Ashton II with 15.9 muM P (S2) and Hoagland with 20 muM P (S3), with or without 50 mM of Tris-HCl buffer (pH 6.5); the control treatment, consisting of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glomus etunicatum; Gigaspora margarita; Scutellospora heterogama; Inoculum production; Nutrient solution.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400004
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Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates BJM
Maia,Leonor C.; Yano-Melo,Adriana M..
The role of the substrate on germination of Gigaspora albida Schenck & Smith was investigated. Spores were desinfested with 0.5% sodium hypochlorite (20 min.) and placed on Petri dishes over a Millipore filter, with one of the following media: a- 1% water-agar; b- water-agar + aqueous extract of roots of Panicum miliaceum L.; c- salt medium of Murashige & Skoog (MS) or sterilized sand; and incubated in the dark at room temperature (28ºC ± 2). The experimental design was at random with four treatments and four replicates. Germination was evaluated every 7 days until the 28th day. The water-agar medium was the most feasible for spore germination at the 7th day, followed by the MS medium at the 14th day. Conversely, the sand and the root...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water-agar; MS medium; Root extract; Glomales; Panicum miliaceum.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400005
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