|
|
|
|
|
Cao,Yuanyuan; Yao,Jianming; Li,Jun; Chen,Xiangsong; Wu,Jinyong. |
To obtain an industrial strain with high lipid yield, the wild strain G0 of Geotrichum robustum was mutated by means of nitrogen ions implantation. Mutagenic effects of strain G0 by low energy N+ ion implantation were studied. The experimental results indicated that the survival rate curve took a ?saddle? shape, and the positive mutation rate was increased to 22.00% at the dose of nitrogen ions 2.0 x 10(15) ions/cm² when the survival rate was 28.60%. By repeated screening, a high lipid producing strain G9 was obtained. The biomass, lipid content and lipid yield of the mutant can reach 40.25 g/L, 71.14% and 28.63 g/L after cultured in a 5L fermenter for 8 days, increasing by 52.81%, 68.82% and 157.93% to those of wild strain, respectively. Analysis results... |
Tipo: Journal article |
Palavras-chave: Fatty acids; Geotrichum robustum; High lipid producing strain; Ion beam implantation. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600002 |
| |
|
|
Yan,Shoubao; Yao,Jianming; Yao,Liming; Zhi,Zhijun; Chen,Xiangsong; Wu,Jingyong. |
The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation of the hydrolysates by Saccharomyces cerecisiae H058 were studied in this work. The optimum batch enzymatic conditions were found to be saccharification pH of 4.5, temperature of 55!, glucoamylase concentration of 120 u/g, α-amylase concentration of 10 u/g, solid-liquid ratio of 1: 0.75 (w/w). Fed batch hydrolysis process was started with a solid-liquid ratio of 1: 1 (w/w), with solid food waste added at time lapse of 2 h to get a final solid-liquid ratio of 1: 0.5 (w/w). After 4 h of reaction, the reducing sugar concentration reached 194.43 g/L with a enzymatic digestibility of 93.12%. Further fermentation of the batch and fed batch enzymatic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food waste; Enzymatic hydrolysis; Hydrolysates; Fermentation; Ethanol production. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200002 |
| |
|
|
|