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Isolation and expression of two polyketide synthase genes from Trichoderma harzianum 88 during mycoparasitism BJM
Yao,Lin; Tan,Chong; Song,Jinzhu; Yang,Qian; Yu,Lijie; Li,Xinling.
Abstract Metabolites of mycoparasitic fungal species such as Trichoderma harzianum 88 have important biological roles. In this study, two new ketoacyl synthase (KS) fragments were isolated from cultured Trichoderma harzianum 88 mycelia using degenerate primers and analysed using a phylogenetic tree. The gene fragments were determined to be present as single copies in Trichoderma harzianum 88 through southern blot analysis using digoxigenin-labelled KS gene fragments as probes. The complete sequence analysis in formation of pksT-1 (5669 bp) and pksT-2 (7901 bp) suggests that pksT-1 exhibited features of a non-reducing type I fungal PKS, whereas pksT-2 exhibited features of a highly reducing type I fungal PKS. Reverse transcription polymerase chain reaction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyketide synthese; Real time quantitative PCR; Gene disruption; Fungal plant pathogens.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200468
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