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The use of non-human primates as animal models for the study of hepatitis viruses BJMBR
Vitral,C.L.; Yoshida,C.F.T.; Gaspar,A.M.C..
Hepatitis viruses belong to different families and have in common a striking hepatotropism and restrictions for propagation in cell culture. The transmissibility of hepatitis is in great part limited to non-human primates. Enterically transmitted hepatitis viruses (hepatitis A virus and hepatitis E virus) can induce hepatitis in a number of Old World and New World monkey species, while the host range of non-human primates susceptible to hepatitis viruses transmitted by the parenteral route (hepatitis B virus, hepatitis C virus and hepatitis delta virus) is restricted to few species of Old World monkeys, especially the chimpanzee. Experimental studies on non-human primates have provided an invaluable source of information regarding the biology and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis viruses; Non-human primates; Animal model; Experimental infection.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800003
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Genotyping hepatitis C virus from hemodialysis patients in Central Brazil by line probe assay and sequence analysis BJMBR
Espírito-Santo,M.P.; Carneiro,M.A.S.; Reis,N.R.S.; Kozlowski,A.G.; Teles,S.A.; Lampe,E.; Yoshida,C.F.T.; Martins,R.M.B..
The present study examined the distribution of hepatitis C virus (HCV) genotypes and subtypes in a hemodialysis population in Goiás State, Central Brazil, and evaluated the efficiency of two genotyping methods: line probe assay (LiPA) based on the 5' noncoding region and nucleotide sequencing of the nonstructural 5B (NS5B) region of the genome. A total of 1095 sera were tested for HCV RNA by RT-nested PCR of the 5' noncoding region. The LiPA assay was able to genotype all 131 HCV RNA-positive samples. Genotypes 1 (92.4%) and 3 (7.6%) were found. Subtype 1a (65.7%) was the most prevalent, followed by subtypes 1b (26.7%) and 3a (7.6%). Direct nucleotide sequencing of 340 bp from the NS5B region was performed in 106 samples. The phylogenetic tree showed that...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Hepatitis C; Hepatitis C virus; Genotypes; Hemodialysis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400013
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Prevalence and risk factors for HBV, HCV and HDV infections among injecting drug users from Rio de Janeiro, Brazil BJMBR
Oliveira,M.L.A.; Bastos,F.I.; Telles,P.R.; Yoshida,C.F.T.; Schatzmayr,H.G.; Paetzold,U.; Pauli,G.; Schreier,E..
Viral hepatitis constitutes a major health issue, with high prevalence among injecting drug users (IDUs). The present study assessed the prevalence and risk determinants for hepatitis B, C and D viruses (HBV, HCV and HDV) infections among 102 IDUs from Rio de Janeiro, Brazil. Serological markers and HCV-RNA were detected by enzyme immunoassay and nested PCR, respectively. HCV genotyping was determined by restriction fragment length polymorphism analysis (RFLP). HBsAg, anti-HBc and anti-HBs were found in 7.8, 55.8 and 24.7% of IDUs, respectively. In the final logistic regression, HBV infection was independently associated with male homosexual intercourse within the last 5 years (odds ratio (OR) 3.1; 95% confidence interval (CI) 1.1-8.8). No subject...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HBV; HDV; HCV; HCV genotypes; IDU.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900009
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Distribution of HCV genotypes among different exposure categories in Brazil BJMBR
Oliveira,M.L.A.; Bastos,F.I.; Sabino,R.R.; Paetzold,U.; Schreier,E.; Pauli,G.; Yoshida,C.F.T..
Hepatitis C virus (HCV) infection is widespread and responsible for more than 60% of chronic hepatitis cases. HCV presents a genetic variability which has led to viral classification into at least 6 genotypes and a series of subtypes. These variants present characteristic geographical distribution, but their association with different responses to treatment with interferon and severity of disease still remains controversial. The aim of this study was to investigate the patterns of distribution of HCV genotypes among different exposure categories in Brazil. Two hundred and fifty anti-HCV positive samples were submitted to HCV-RNA detection by RT-PCR and their genotype was determined by restriction fragment length polymorphism (RFLP) analysis. In addition,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: HCV; HCV genotypes; Brazil.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300005
Registros recuperados: 4
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