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	Provedor de dados Electron. J. Biotechnol. (1) Autor Fang,Baishan (1) Li,Wei (1) Ng,I-Son (1) Yu,Jincong (1) Zhang,Guangya (1) Palavra-chave 1 (1) 3-propanediol oxidoreductase codon optimization enzymatic properties Escherichia coli overlap extension PCR (1) Tipo do documento Journal article (1) Ano 2011 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Codon optimization of 1,3-propanediol oxidoreductase expression in Escherichia coli and enzymatic properties Electron. J. Biotechnol. Li,Wei; Ng,I-Son; Fang,Baishan; Yu,Jincong; Zhang,Guangya. The gene dhaT from Klebsiella pneumoniae encoding 1,3-propanediol oxidoreductase (PDOR) was de novo synthesized by splicing overlap extension polymerase chain reaction (SOE-PCR) primarily according to Escherichia coli’s codon usage, as well as mRNA secondary structure. After optimization, Codon Adaptation Index (CAI) value was improved from 0.75 to 0.83, meanwhile energy of mRNA secondary structure was increased from -400.1 to -86.8 kcal/mol. This synthetic DNA was under control by phage T7 promoter in the expression vector pET-15b and transformed into the E. coli BL21 (DE3) strain. Inducers such as isopropyl β-D-thiogalactoside (IPTG) and lactose were compared by activity at different inducing time. The activity of PDOR after codon optimized was... Tipo: Journal article Palavras-chave: 1; 3-propanediol oxidoreductase codon optimization enzymatic properties Escherichia coli overlap extension PCR. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400007 Registros recuperados: 1 Primeira ... 1 ... Última

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