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	Provedor de dados Biocell (2) Autor Doncel,Gustavo F. (2) Yunes,Roberto (2) Acosta,Anibal A. (1) Acosta,Aníbal A. (1) Fernández,Pedro (1) Palavra-chave Sperm motility (2) Assisted reproduction (1) Asthenozoospermia (1) Hyperactivation (1) PDE inhibition (1) Phosphotyrosine proteins (1) Protein-tyrosine kinase (1) Sperm hyperactivation (1) Tyrosine-phosphorylation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (1) 2003 (1) País Argentina (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cyclic nucleotide phosphodiesterase inhibition increases tyrosine phosphorylation and hyper motility in normal and pathological human spermatozoa Biocell Yunes,Roberto; Fernández,Pedro; Doncel,Gustavo F.; Acosta,Aníbal A.. Our objective was to determine the effect of phosphodiesterase (PDE) inhibition on: 1) tyrosine phosphorylation of human spermatozoa at the tail level; and 2) sperm motion parameters and hyperactivated motility. The study was conducted with normozoospermic and asthenozoospermic samples incubated under in vitro capacitating conditions. The main outcome measures were computer-assisted sperm motion analysis and fluorescent immunodetection of phosphotyrosine-containing proteins. Pentoxifylline (PTX) was used as PDE inhibitor because of its wide use in the clinic. PTX-treatment significantly increased sperm velocity, hyperactivated motility and tyrosine-phosphorylation, both in normo and asthenozoospermic samples. Tyrosine-phosphorylation of tail proteins was... Tipo: Info:eu-repo/semantics/article Palavras-chave: PDE inhibition; Phosphotyrosine proteins; Sperm hyperactivation; Sperm motility; Assisted reproduction. Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300006 Incidence of sperm-tail tyrosine phosphorylation and hyperactivated motility in normozoospermic and asthenozoospermic human sperm samples Biocell Yunes,Roberto; Doncel,Gustavo F.; Acosta,Anibal A.. Our objective was to study the incidence of sperm-tail phosphotyrosine immunoreactivity in normozoospermic and asthenozoospermic human sperm samples, its association with sperm motion parameters, particularly hyperactivated motility, and its potential involvement in the pathogenesis of asthenozoospermia. The work was conducted as a prospective experimental study in the Sperm Biology and Andrology laboratories of the Jones Institute, a medical school-based fertility center. The study subjects were healthy fertile male donors (normozoospermic samples) and infertile patients (asthenozoospermic samples) attending the center. Recently ejaculated semen samples were washed twice to eliminate seminal plasma and a swim-up was performed to select the motile... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sperm motility; Hyperactivation; Asthenozoospermia; Tyrosine-phosphorylation; Protein-tyrosine kinase. Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100004 Registros recuperados: 2 Primeira ... 1 ... Última

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