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Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine Anais da ABC (AABC)
PEREIRA,RENATA V.F.; LINDEN,DAVID R.; MIRANDA-NETO,MARCÍLIO H.; ZANONI,JACQUELINE N..
ABSTRACT The objective of this study was to investigate the effects of 2% L-glutamine supplementation on myenteric innervation in the ileum of diabetic rats, grouped as follows: normoglycemic (N); normoglycemic supplemented with L-glutamine (NG); diabetic (D); and diabetic supplemented with L-glutamine (DG). The ileums were subjected to immunohistochemical techniques to localize neurons immunoreactive to HuC/D protein (HuC/D-IR) and neuronal nitric oxide synthase enzyme (nNOS-IR) and to analyze varicosities immunoreactive to vasoactive intestinal polypeptide (VIP-IR) and calcitonin gene-related peptide (CGRP-IR). L-Glutamine in the DG group (i) prevented the increase in the cell body area of nNOS-IR neurons, (ii) prevented the increase in the area of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetic neuropathy; Glutamine; Ileum; Myenteric innervation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200609
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Effect of l-glutamine on myenteric neuron and of the mucous of the ileum of diabetic rats Anais da ABC (AABC)
TRONCHINI,ELEANDRO A.; TREVIZAN,ALINE R.; TASHIMA,CRISTIANO M.; FREITAS,PRISCILA DE; BAZOTTE,ROBERTO B.; PEREIRA,MARLI A.S.; ZANONI,JACQUELINE N..
The objective of this work was to investigate the effect of the L-glutamine supplementation to prevent - diabetes induced changes in myenteric neurons and also to verify the effect on the mucosa of the ileum of Wistar rats. The animals were divided in five groups (n = 5): untreated normoglycaemic (UN), normoglycaemic treated with L-glutamine (NG), untreated diabetics (UD), diabetics treated with L-glutamine, starting on the 4th (DG4) or 45thday following diabetes induction (DG45). The amino acid was added to the diet at 1%. The density and size of neurons, the metaphasic index in the crypt, the height of the villus, the depth of the crypt and the number of globet cells were determined. There was no difference in the neuronal density and in the cellular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetes mellitus; L-glutamine; Myenteric plexus; Myosin-V; Rats.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301165
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