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| Brasileiro-Vidal,Ana Christina; Cuadrado,Angeles; Brammer,Sandra P.; Zanatta,Ana Christina A.; Prestes,Ariano M.; Moraes-Fernandes,Maria Irene B.; Guerra,Marcelo. |
| Thinopyrum ponticum (2n = 10x = 70, JJJJsJs) belongs to the Triticeae tribe, and is currently used as a source of pathogen resistance genes in wheat breeding. In order to characterize its chromosomes, the number and position of 45S and 5S rDNA sites, as well as the distribution of the repetitive DNA sequences pAs1 and pSc119.2, were identified by fluorescent in situ hybridization. The number of nucleoli and NORs was also recorded after silver nitrate staining. Seventeen 45S and twenty 5S rDNA sites were observed on the short arms of 17 chromosomes, the 45S rDNA was always located terminally. On three other chromosomes, only the 5S rDNA site was observed. Silver staining revealed a high number of Ag-NORs (14 to 17) on metaphase chromosomes, whereas on... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thinopyrum; Silver staining; 45S and 5S rDNA; PSc119.2; PAs1. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400014 |
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| Fernandes,Maria Irene Baggio de Moraes; Zanatta,Ana Christina A.; Prestes,Ariano Moraes; Caetano,Vanderlei da Rosa; Barcellos,Amarilis Labes; Angra,Dilma Cristina; Pandolfi,Valesca. |
| Worldwide wheat (Triticum aestivum L. em. Thell, 2n = 6X = 42, AABBDD) breeding programs aim to reorganize genotypes to achieve better yields, environmental adaptation and food quality. The necessary interdisciplinarity for breeding purposes requires an accurate choice of the most appropriate cellular and/or molecular strategies available to be integrated with agronomic approaches in order to overcome the genetic limitation of each cultivated species, at each agroecosystem. Cytogenetics has given a great contribution to wheat genetic studies and breeding, due to viability of chromosomal variants because of homoeology among genomes in this allohexaploid species and the genus Triticum. The level of development of cytogenetic techniques achieved over the last... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400051 |
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