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| Traugott, Michael; Zangerl, Petra; Juen, Anita; Schallhart, Nikolaus; Pfiffner, Lukas. |
| The use of diagnostic polymerase chain reaction (PCR) is a valuable approach to study host–parasitoid interactions. It circumvents problems inherent to rearing parasitoids from Weld-collected hosts or host dissection. Here, we present a PCR-based detection method for Diadegma semiclausum, Cotesia glomerata, and Cotesia rubecula, which are key parasitoids of the three lepidopteran cabbage pests Plutella xylostella, Pieris brassicae, and Pieris rapae, respectively. Primer pairs for the three parasitoid species and Pl. xylostella were developed: they were used either separately in singleplex PCR or combined in multiplex PCR to (1) screen simultaneously for the two Cotesia species or to (2) detect the parasitoid D. semiclausum and identify its host Pl.... |
| Tipo: Journal paper |
Palavras-chave: Crop health; Quality; Protection. |
| Ano: 2006 |
URL: http://orgprints.org/13143/1/Traugott%2Detal%2D2006%2Dbiocontrol%2Dbiol%2DControl.pdf.pdf |
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