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	Provedor de dados Electron. J. Biotechnol. (2) Autor López Bilbao,Marisa (2) López,Nilda (2) Radonic,Laura Mabel (2) Zavallo,Diego (2) Zimmermann,Julián Marcos (2) Palavra-chave Sunflower (2) Antifungal genes (1) Double expression cassettes (1) Genetic transformation (1) Kanamycin (1) Polyprotein cassettes (1) Selection (1) Transgenic plants (1) Mais... Tipo do documento Journal article (2) Ano 2008 (1) 2006 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Rooting in Km selective media as efficient in vitro selection method for sunflower genetic transformation Electron. J. Biotechnol. Radonic,Laura Mabel; Zimmermann,Julián Marcos; Zavallo,Diego; López,Nilda; López Bilbao,Marisa. Despite of numerous publications in sunflower genetic transformation, there is no efficient or reproducible protocol with low number of escapes. The latter would indicate that the selection method is not effective. In this work we used Km as selective agent, Agrobacterium tumefaciens EHA105 strain and a vector with the nptII gene under the nos promoter and uidA gene under 35S promoter. The response of agroinfected (A) and control (C) explants during the in vitro culture was studied and in both cases in presence or absence of Km in order to assign a differential morphologic response between transformed and non-transformed plants. The characteristics analyzed were: height, colour/aspect of the plantlets, in vitro rooting and in vitro bud-flower development.... Tipo: Journal article Palavras-chave: Genetic transformation; Kanamycin; Selection; Sunflower. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300027 Introduction of antifungal genes in sunflower via Agrobacterium Electron. J. Biotechnol. Radonic,Laura Mabel; Zimmermann,Julián Marcos; Zavallo,Diego; López,Nilda; López Bilbao,Marisa. There is evidence that overexpression of transgenes codifying antifungal proteins may confer protection to pathogen attack, and that this protection is increased due to the synergic effect of the expression of two or more genes. On the other hand it is well known that sunflower is a recalcitrant specie, highly difficult to be genetically transformed. In this context, the final aim of this project was to obtain sunflower plants expressing at least two antifungal genes, avoiding sequential transformation. The antifungal genes used encode for two enzymes that degrade the fungal wall (glucanase and chitinase), an osmotin and a ribosome inhibitor protein. Two types of transformation vectors were used: a more traditional system with a double cassette and a novel... Tipo: Journal article Palavras-chave: Antifungal genes; Double expression cassettes; Polyprotein cassettes; Sunflower; Transgenic plants. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500008 Registros recuperados: 2 Primeira ... 1 ... Última

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