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Rafal Woycicki; Wojciech Gutman; Zbigniew Przybecki. |
Analyzing of sequences similarities is the first and most important method used to find out the function of unknown nucleotides. Searching of homologs should be done carefully not to loose any important ones. Having thousands of results from various long-read sequencing projects (ie. differentially expressed tags, genomic polymorphons or BAC ends), the by-hand ability to retrieve interesting (to our goal) similarities in hundreds of Blast results decreases rapidly. Decreasing the number of retrieved sequences by giving more stringency in e-value threshold or displaying less results could lead to false deductions. Functional genomics, proteomics and metabolomics could give us answers to the role of nucleotide sequences. It makes the need to annotate as much... |
Tipo: Poster |
Palavras-chave: Genetics & Genomics; Bioinformatics; Plant Biology. |
Ano: 2009 |
URL: http://precedings.nature.com/documents/3900/version/1 |
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Rafal Woycicki; Zbigniew Przybecki. |
Genome sequencing give us an opportunity to broaden the range and improve the quality of omics studies. New sequencing technologies make it possible to achieve the sequencing reads fast and cheap. Since the assembly step of such next generation reads is still not well standardized it is the most cumbersome part of sequencing projects.
We focus here on the wide range of approaches undertaken to assemble the draft cucumber (Cucumis sativus L. cv. Borszczagowski) genome done by using 12x coverage of pyrosequencing Titanium chemistry shotgun paired and unpaired reads combined with Sanger reads. |
Tipo: Poster |
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Plant Biology. |
Ano: 2010 |
URL: http://precedings.nature.com/documents/4630/version/1 |
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