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	Provedor de dados Electron. J. Biotechnol. (1) Genet. Mol. Biol. (1) Autor Zhang,Daoxiang (2) Bao,Lumin (1) Gai,Yunchao (1) Peihan,Li (1) Song,Linsheng (1) Wang,Lingling (1) Wang,Mengqiang (1) Xiang,Taihe (1) Zhang,Huan (1) Zhou,Zhi (1) Mais... Palavra-chave Antifungal activity (1) Benzene (1) CYP2E1 gene (1) Cyclophilin A (1) Eriocheir sinensis (1) Formaldehyde (1) Immune response (1) PPIase activity (1) Petunia hybrida (1) Toluene (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2013 (1) 2011 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein Electron. J. Biotechnol. Wang,Mengqiang; Zhang,Daoxiang; Wang,Lingling; Gai,Yunchao; Zhou,Zhi; Zhang,Huan; Song,Linsheng. Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities. Results: The full-length cDNA of crab Eriocheir sinensis CypA (EsCypA) was cloned by EST and RACE technique. The complete sequence of EsCypA cDNA contained a 5' untranslated region (UTR) of 50 bp, a 3’ UTR of 233 bp with a polyA tail, and an open reading frame (ORF) of 495 bp encoding a polypeptide of 164 amino acids with the predicted molecular weight of 17.36 kDa. The deduced amino acid sequence of EsCypA contained two highly conserved signature sequences of... Tipo: Journal article Palavras-chave: Antifungal activity; Cyclophilin A; Eriocheir sinensis; Immune response; PPIase activity. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200002 Transgenic plants of Petunia hybrida harboring the CYP2E1 gene efficiently remove benzene and toluene pollutants and improve resistance to formaldehyde Genet. Mol. Biol. Zhang,Daoxiang; Xiang,Taihe; Peihan,Li; Bao,Lumin. The CYP2E1 protein belongs to the P450 enzymes family and plays an important role in the metabolism of small molecular and organic pollutants. In this study we generated CYP2E1 transgenic plants of Petunia using Agrobacterium rhizogenes K599. PCR analysis confirmed that the regenerated plants contained the CYP2E1 transgene and the rolB gene of the Ri plasmid. Southern blotting revealed the presence of multiple copies of CYP2E1 in the genome of transgenic plants. Fluorescent quantitative PCR revealed exogenous CYP2E1 gene expression in CYP2E1 transgenic plants at various levels, whereas no like expression was detected in either GUS transgenic plants or wild-types. The absorption of benzene and toluene by transgenic plants was analyzed through quantitative... Tipo: Info:eu-repo/semantics/article Palavras-chave: Petunia hybrida; CYP2E1 gene; Benzene; Toluene; Formaldehyde. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400016 Registros recuperados: 2 Primeira ... 1 ... Última

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