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Impact of the tissue factor pathway inhibitor gene on apoptosis in human vascular smooth muscle cells Genet. Mol. Biol.
Dong,Xia; Song,Li-ping; Zhu,Dun-wan; Zhang,Hai-ling; Liu,Lan-xia; Leng,Xi-gang.
Tissue factor pathway inhibitor (TFPI) plays a vitally important role in the blood coagulation pathway. Recent studies indicated that TFPI induces apoptosis in vascular smooth-muscle cells (VSMCs) in animals. The present study investigated whether the TFPI gene could also induce apoptosis in human vascular smooth-muscle cells (hVSMCs). Such cells were isolated from human umbilical arteries and subsequently transfected with pIRES-TFPI plasmid (2 μg/mL). MTT assaying and cell counting were applied to measure cell viability and proliferation, RT-PCR was utilized to analyze TFPI gene expression in the cells. Apoptosis was analyzed by fluorescence activated cell sorting (FACS). Several key proteins involved in apoptosis were examined through Western blotting....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue factor pathway inhibitor; Vascular smooth muscle cells; Apoptosis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100005
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