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| Zhou,Huiqin; Du,Hong; Zhang,Haifang; Shen,Haiying; Yan,Ruhong; He,Yun; Wang,Min; Zhu,Xueming. |
| Staphylococcus aureus (S. aureus) is an important human pathogen, which commonly causes the acquired infectious diseases in the hospital and community. Effective and simple antibiotic treatment against S. aureus-related disease becomes increasingly difficult. Developing a safe and effective vaccine against S. aureus has become one of the world's hot spots once again. The key issue of developing the vaccine of S. aureus is how to find an ideal key pathogenic gene of S. aureus. It was previously suggested that EsxA might be a very important factor in S. aureus abscess formation in mice, but clinical experimental evidence was lacking. We therefore expressed EsxA protein through prokaryotic expression system and purified EsxA protein by Ni-affinity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: S. aureus; EsxA; Anti-EsxA antibodies; Multi-drug resistant. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100038 |
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| Xu,Shungao; Zou,Xin; Sheng,Xiumei; Zhang,Haifang; Mao,Lingxiang; Du,Hong; Xu,Huaxi; Huang,Xinxiang. |
| Salmonella enterica serovar Typhi z66-positive strains have two different flagellin genes, fliC:d/j and fljB:z66, located on the chromosome and on a linear plasmid, respectively. To investigate the mechanism underlying the expressional regulation of fljB:z66, gene deletion mutants of the regulators FliA, FlhDC, and OmpR were constructed in this study. The expression levels of fliC and fljB:z66 were analyzed by qRT-PCR in the wild-type strain and mutants at high and low osmolarity. The results show that the expression levels of both fljB:z66 and fliC were greatly reduced in fliA and flhDC mutants under both high and low osmotic conditions. In the ompR mutant, the expression levels of fljB:z66, fliC, fliA, and flhD were increased at low osmotic conditions.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella enterica serovar Typhi; FljB:z66; FlhDC; FliA; OmpR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300025 |
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