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Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China BJID
Yuan,Kai; Zhong,Zhao-ming; Zhang,Qiang; Xu,Shu-chai; Chen,Jian-ting.
BACKGROUND: Atypical spinal tuberculosis (TB) usually presents in a slowly indolent manner with nonspecific clinical presentations making the diagnosis a great challenge for physicians. New technologies for the detection of atypical spinal TB are urgently needed. The aim of this study was to assess the diagnostic value of an enzyme-linked immunospot (ELISPOT) assay in clinically suspected cases of atypical spinal TB in China. METHODS: From March 2011 to September 2012, a total of 65 patients with suspected atypical spinal TB were enrolled. In addition to conventional tests for TB, we used ELISPOT assays to measure the IFN-I response to ESAT-γ and CFP-10 in T-cells in samples of peripheral blood mononuclear cells. Patients with suspected atypical spinal TB...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atypical spinal tuberculosis; Enzyme-linked immunospot assay; T-SPOT.TB kit; Diagnosis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000500004
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Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity BJM
Gong,Tianxiang; Li,Wanyi; Wang,Yueling; Jiang,Yan; Zhang,Qiang; Feng,Wei; Jiang,Zhonghua; Li,Mingyuan.
Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fermentation parameters: induce with 0.6 mM isopropylthiogalactoside (IPTG) at 34ºC in 2×YT medium and harvest at 6 h postinduction, fusion protein TrxA-mBD2 was high expressed in the soluble fraction (>95%). After cleaved fusion protein by enterokinase, soluble mature mBD2 was achieved 6 mg/L with a volumetric productivity. Purified recombinant mBD2 demonstrated clear broad-spectrum antimicrobial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mouse beta defensin 2; Expression; Purification; Antimicrobial activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300043
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Late nitrogen application enhances spikelet number in indica hybrid rice (Oryza sativa L.) Scientia Agricola
Zhou,Wei; Lv,Tengfei; Chen,Yong; Hu,Jianfeng; Zhang,Qiang; Ren,Wanjun.
ABSTRACT To increase rice yield potential, field experiments were conducted in farmers’ paddies in 2011 and 2012 to evaluate the effects of different nitrogen applications on the yield and panicle components of three typical indica hybrid rice varieties in Sichuan Province. The number of grains per panicle resulting from late nitrogen application (LA) was 12 % greater than that obtained from traditional nitrogen application (TA); this increase was the main source of improvements in yield. The number of surviving and differentiated spikelets (NSS and NDiS) resulting from LA was significantly higher than that measured under TA, especially for the Fyou498 cultivar, where the NSS and NDiS increased by 15 % and 14 %, respectively. Compared with TA, the number...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Panicle; Grain number; Branch; Degenerated rate; Yield.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200127
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