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	Provedor de dados BJID (1) BJPS (1) Autor Zhang,Xiaoxiao (2) Bi,Wenzi (1) Cao,Guangxiang (1) Cao,Jianming (1) Chen,Lijiang (1) Chen,Yuguo (1) Dong,Guofeng (1) Jin,Xiaoqing (1) Li,Jiahui (1) Liu,Haiyang (1) Wang,Liang (1) Zhi,Xiaoyang (1) Zhong,Chuanqing (1) Zhou,Tieli (1) Mais... Palavra-chave Biofilm formation (1) Ciprofloxacin (1) Clavulanic acid (1) Escherichia coli (1) High Performance Liquid Chromatography-Mass Spectrometry-Mass Spectrometry (1) Potassium clavulanate (1) Related substance G (1) Sub-minimum inhibitory concentration (1) Virulence (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2015 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effects of sub-minimum inhibitory concentrations of ciprofloxacin on biofilm formation and virulence factors of Escherichia coli BJID Dong,Guofeng; Li,Jiahui; Chen,Lijiang; Bi,Wenzi; Zhang,Xiaoxiao; Liu,Haiyang; Zhi,Xiaoyang; Zhou,Tieli; Cao,Jianming. ABSTRACT Objective: To evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates. Methods: Sub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922. Results:... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm formation; Ciprofloxacin; Escherichia coli; Sub-minimum inhibitory concentration; Virulence. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100015 Studies on the formation and synthetic mechanism of related substance G in potassium clavulanate production BJPS Jin,Xiaoqing; Cao,Guangxiang; Zhang,Xiaoxiao; Chen,Yuguo; Wang,Liang; Zhong,Chuanqing. The objective of this study was to investigate the formation and synthetic mechanism of related substance G in potassium clavulanate production. The impurity in the potassium clavulanate final product, with a retention time of 13.5 min, was confirmed as related substance G by high performance liquid chromatography-mass spectrometry/mass spectrometry. Related substance G analysis during the production of clavulanic acid showed that this impurity could be synthesized during fermentation, and the amount increased with the fermentation time. Studies on its synthetic mechanism showed that L-tyrosine and succinic acid were the precursors for biosynthesis of related substance G in vivo. The reaction was deduced to be catalyzed by an enzyme. The enzyme was a type... Tipo: Info:eu-repo/semantics/article Palavras-chave: Potassium clavulanate; Clavulanic acid; Related substance G; Related substance G; High Performance Liquid Chromatography-Mass Spectrometry-Mass Spectrometry. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100077 Registros recuperados: 2 Primeira ... 1 ... Última

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