Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJPS (1) Electron. J. Biotechnol. (1) Autor Zhang,Yue (2) Chen,Peng (1) Fu,Ting (1) He,Bo (1) Li,Aiqin (1) Long,Jiang (1) Shen,Zhiqiang (1) Wang,Xingjun (1) Xia,Han (1) Yang,Renhua (1) Yuan,Mei (1) Zhang,Xiaochao (1) Zhao,Chuanzhi (1) Mais... Palavra-chave Arachis hypogaea L. (1) CDNA library (1) Chalcone isomerase (1) Expression analysis (1) Geraniin/effect (1) Osteoblasts (1) Osteoprotegerin (1) Receptor activator of nuclear factor -κ B ligand. (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2018 (1) 2012 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A novel effect of geraniin on OPG/RANKL signaling in osteoblasts BJPS Zhang,Xiaochao; Yang,Renhua; Long,Jiang; He,Bo; Zhang,Yue; Fu,Ting; Shen,Zhiqiang; Chen,Peng. In this study, the effects of geraniin on osteoprotegerin/receptor activator of nuclear factor-κB ligand(OPG/RANKL) in regulating the proliferation of osteoblasts and suppression of osteoclast-like cells (OLC) in OLC-osteoblast co-cultured system in vitro were investigated. Osteoblasts were cultured and identified with alkaline phosphatase (ALP), gomori stain, and mineralized nodule stain. OLCs were isolated from long bones of Sprague-Dawley (SD) rats and identified with tartrate-resistant acid phosphatase(TRAP) stain. Methyl thiazolyl tetrazolium assay was used to examine the proliferation of osteoblasts, and immunocytochemistry and in situ hybridization to analyze the expression OPG/RANKL in osteoblasts co-cultured with osteoclasts under the action of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Geraniin/effect; Osteoblasts; Osteoprotegerin; Receptor activator of nuclear factor -κ B ligand.. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300618 Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene Electron. J. Biotechnol. Zhang,Yue; Xia,Han; Yuan,Mei; Zhao,Chuanzhi; Li,Aiqin; Wang,Xingjun. Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than... Tipo: Journal article Palavras-chave: Arachis hypogaea L.; CDNA library; Chalcone isomerase; Expression analysis. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco