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| Yang,Weichao; Han,Litao; Mandlaa,Mandlaa; Zhang,Haihong; Zhang,Zhongze; Xu,Hui. |
| Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining positive mutants. The proposed plate screening method is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ketogulonicigenium vulgare; PH indicator; Rapid screening; Spaceflight mutation; 2-Keto-l-gulonic acid. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300397 |
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