|
|
|
|
|
| Mo,Fei; Zhao,Jie; Liu,Na; Cao,Li-hua; Jiang,Shan-xiang. |
| Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across different tissue types or under different experimental conditions. To identify the most suitable reference genes for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus) under various conditions (sex, age, water temperature, and drug treatments), seven reference genes, including beta actin (ACTB), beta-2 microglobulin (B2M), embryonic elongation factor-1 alpha (EEF1A), glyceraldehyde phosphate dehydrogenase (GAPDH), alpha tubulin (TUBA), ribosomal protein l8 (RPL8) and glucose-6-phosphate dehydrogenase (G6PDH), were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Quantitative RT-PCR; Housekeeping genes; CYP4T; Crucian carp. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400005 |
| |
|
|
| Zhang,Yafei; Zhang,Bicheng; Zhang,Anran; Li,Xiaohua; Liu,Jian; Zhao,Jie; Zhao,Yong; Gao,Jianfei; Fang,Dianchun; Rao,Zhiguo. |
| A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MiR-26a; IL-6; C-Myc; Hepatocellular carcinoma; Carcinoma prognosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032 |
| |
|
|
|
