Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJMBR (1) Autor Hou,Yayi (1) Huang,Yahong (1) Xie,Hao (1) Zhao,Guangfeng (1) Zhao,Shuli (1) Palavra-chave Doxorubicin (1) Midkine (1) Single-chain variable fragments (1) Tumor targeting (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2012 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última A conjugate of an anti-midkine single-chain variable fragment to doxorubicin inhibits tumor growth BJMBR Zhao,Shuli; Zhao,Guangfeng; Xie,Hao; Huang,Yahong; Hou,Yayi. Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) was used to target the chemotherapeutic agent to a mouse solid tumor model in which the tumor cells expressed high levels of human MK. The His-tagged recombinant scFv was expressed in bacteria, purified by metal affinity chromatography, and then conjugated to DOX using oxidative dextran (Dex) as a linker. The molecular formula of this immunoconjugate was scFv(Dex)1.3(DOX)20. In vitro apoptosis assays showed that the scFv-DOX conjugate was more cytotoxic against MK-transfected human adenocarcinoma cells (BGC823-MK) than untransfected cells (55.3 ± 2.4 vs 22.4 ± 3.8%) for three independent experiments. Nude mice bearing... Tipo: Info:eu-repo/semantics/article Palavras-chave: Single-chain variable fragments; Midkine; Tumor targeting; Doxorubicin. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300008 Registros recuperados: 1 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco