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| Mosimann, Carla; Oberhänsli, Thomas; Ziegler, Dominik; Nassal, Dinah; Kandeler, Ellen; Boller, Thomas; Mäder, Paul; Thonar, Cécile. |
| TaqMan-based quantitative PCR (qPCR) assays were developed to study the persistence of two well-characterized strains of plant growth-promoting rhizobacteria (PGPR), Pseudomonas fluorescens Pf153 and Pseudomonas sp. DSMZ 13134, in the root and rhizoplane of inoculated maize plants. This was performed in pot experiments with three contrasting field soils (Buus, Le Caron and DOK-M). Potential cross-reactivity of the qPCR assays was assessed with indigenous Pseudomonas and related bacterial species, which had been isolated from the rhizoplane of maize roots grown in the three soils and then characterized by Matrix-Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry (MS). Sensitivity of the qPCR expressed as detection limit of... |
| Tipo: Journal paper |
Palavras-chave: Soil biology Crop health; Quality; Protection. |
| Ano: 2017 |
URL: http://orgprints.org/31294/1/mosimann-etal-2017-fmicb-vol7-article02150.pdf |
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| Mathu Ndungu, Samuel; Messmer, Monika; Ziegler, Dominik; Gamper, Hannes; Mészáros, Éva; Thuita, Moses; Vanlauwe, Bernard; Frossard, Emmanuel; Thonar, Cécile. |
| Cowpea (Vigna unguiculata L. Walp.) is an important African food legume suitable for dry regions. It is the main legume in two contrasting agro-ecological regions of Kenya as an important component of crop rotations because of its relative tolerance to unpredictable drought events. This study was carried out in an effort to establish a collection of bacterial root nodule symbionts and determine their relationship to physicochemical soil parameters as well as any geographical distributional patterns. Bradyrhizobium spp. were found to be widespread in this study and several different types could be identified at each site. Unique but rare symbionts were recovered from the nodules of plants sampled in a drier in-land region, where there were also overall more... |
| Tipo: Journal paper |
Palavras-chave: Vegetables Crop health; Quality; Protection Africa. |
| Ano: 2018 |
URL: http://orgprints.org/33099/1/Ndungu-etal-2018-AEE-Vol261-p161-171.pdf |
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