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Distribution of biglycan and decorin in rat dental tissue BJMBR
Tenório,D.M.H.; Santos,M.F.; Zorn,T.M.T..
Biglycan and decorin are small leucine-rich proteoglycans that play several biological and structural roles in different tissues and organs. Several reports have indicated that biglycan participates in odontoblast and ameloblast differentiation and in the calcification process. In the present study we show that the expression of biglycan changes from within the ameloblasts and odontoblasts to the extracellular space according to the stage of animal development. In predentin and in the pulp space, however, biglycan was continually expressed throughout the period of investigation. In contrast, decorin was absent in odontoblasts and in ameloblasts and was exclusively expressed in predentin throughout the period of observation. In young rats, however, decorin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decorin; Biglycan; Odontoblasts; Predentin; Immunohistochemistry.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800012
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Distribution of versican and hyaluronan in the mouse uterus during decidualization BJMBR
San Martin,S.; Soto-Suazo,M.; Zorn,T.M.T..
Preparation for embryo implantation requires extensive adaptation of the uterine microenvironment. This process consists of cell proliferation and cell differentiation resulting in the transformation of endometrial fibroblasts into a new type of cell called decidual cell. In the present study, we followed the space-time distribution of versican and hyaluronan (HA) in different tissues of the uterus before and after embryo implantation. Fragments of mouse uteri obtained on the fourth, fifth, sixth and seventh days of pregnancy were fixed in Methacarn, embedded in Paraplast and cut into 5-µm thick sections. HA was detected using a biotinylated fragment of the proteoglycan aggrecan, which binds to this glycosaminoglycan with high affinity and specificity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decidualization; Endometrium; Proteoglycans; Versican; Hyaluronan; Mouse.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800013
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Autoradiography reveals regional metabolic differences in the endometrium of pregnant and nonpregnant mice BJMBR
Oliveira,S.F.; Abrahamsohn,P.; Zorn,T.M.T..
The rodent endometrium undergoes remarkable modifications during pregnancy, resulting from a redifferentiation of its fibroblasts. During this modification (decidualization), the fibroblasts transform into large, polyhedral cells that establish intercellular junctions. Decidualization proceeds from the subepithelial stroma towards the deep stroma situated next to the myometrium and creates regions composed of cells in different stages of differentiation. We studied by autoradiography whether cells of these different regions have different levels of macromolecular synthesis. Radioactive amino acids or radioactive sulfate were administered to mice during estrus or on different days of pregnancy. The animals were killed 30 min after injection of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autoradiography lsqb; 3Hrsqb; -proline lsqb; 35Srsqb; -sulfate endometrium mouse.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200015
Registros recuperados: 3
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