| Twenty-seven ejaculates of an adult, mixed breed, medium size dog were used in this study. The semen was collected by penile stimulation and divided into three equal samples. Group I (G I): cooled without extender; Group 2 (G2): diluted with skimmed milk-based extender; Group 3 (G3): diluted with glycine egg yolk-based extender, without glycerol. Samples were evaluated after collection (MO) as to sperm motility and vigor, and integrity of sperm membranes, and then incubated at 50 C. Samples were again evaluated for motility and vigor after being refrigerated for 24 (M 1),48 (M2) and 72 (M3) hours. M3 also included the integrity of sperm membranes in the evaluation. Statistical analysis of the obtained data showed G2 and G3 as equivalent, both being... |
