| With the purpose of obtaining embryogenic calluses, different auxins and inoculation position of the explants were evaluated. Rhizomes of about 0.5cm obtained from micropropagated seedlings were cut lengthwise at half and inoculated in test tubes. After inoculation, the cultures were kept in growth room at 25±2ºC in the dark. The completely randomized design in a factorial scheme 6x2, represented by five auxins (ANA; AIB; AIA; 2,4-D and picloram) at the concentration of 10µM and a control (without auxin) and two explant positions (H-horizontal and V-vertical). The experiment was constituted of five replicates with three tubes each containing one explant. After 50 days’ cultivation, both the percentage of oxidation and callus formation were evaluated. The... |
