| The morphological characteristics of the hooves of two groups of horses were studied. The first group was comprised of six adult animals without any treatment and served as the control. The other group, comprised of seven horses, received carbohydrates overload orally. Of these, 71% developed laminitis between 36 and 48 hours after carbohydrate administration. After 48 hours of induction, the animals were sacrificed and their fore limbs and hindlimbs were removed for histologic study. Tissue sections 5 to 7 µm thick were obtained, stained with hematoxylin-eosin (H&E) or periodic acid Schiff's (PAS), and then examined by light microscopy. It was possible to identify in horses from the laminitis group, morphological and structural changes in the... |
