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The role of biotechnology in agricultural production and food supply Ciencia e Investigación Agraria
Cano Estrada,Araceli; Vélez Díaz,Daniel; Morgado Hernández,Carlos Alberto.
Over the past several decades, technological developments and modernization have grown concomitantly.For example, advances in biotechnology have been used as a tool to increase food production. Specifically, advances in genetic engineering have made possible the manipulation of crops to increase yield, guaranteeing food supplies for the increasing world population. However, transgenic crops have not been well received by all members of society, and there is still uncertainty about their social benefits and the possible implications to human health. Additionally, the benefits of agricultural modernization have favored only developed countries, whereas people living in developing and underdeveloped countries suffer rampant hunger, malnutrition and poverty....
Tipo: Journal article Palavras-chave: Agriculture; Development; Food industry; Nutrition; Organic food; Transgenic food.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000100001
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Evaluation of the use of wound-protectant fungicides and biological control agents against stem canker (Neofusicoccum parvum) of blueberry Ciencia e Investigación Agraria
Latorre,Bernardo A; Torres,René; Silva,Tatiane; Elfar,Karina.
B.A. Latorre, R. Torres, T. Silva, and K. Elfar. 2013. Evaluation of the use of wound-protectant fungicides and biological control agents against stem canker (Neofusicoccum parvum) of blueberry. Cien. Inv. Agr. 40(3): 537-545. Economically, blueberry (Vaccinium corymbosum) has become a very important fruit crop in Chile, and stem canker (Neofusicoccum parvum) has frequently been observed as a major disease. The symptoms are characterized by partial or total death of the foliage associated with extensive reddish-brown canker lesions at the base of the stems. Pruning wounds appear to be the main infection route. In this study, fungicide pastes and biological control agents were evaluated for their effectiveness as pruning wound-protectants against N. parvum....
Tipo: Journal article Palavras-chave: Botryosphaeria canker; Biological control; Boric acid; Ascorbic acid; Vaccinium corymbosum.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300007
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Variabilidad en el florecimiento de procedencias de Eucalyptus cladocalyx en la Región de Coquimbo Ciencia e Investigación Agraria
Mora,Freddy; Perret,Sandra; Scapim,Carlos Alberto; Nunes Martins,Elias; Molina,María Paz.
Se analizó la variabilidad en el florecimiento de cinco poblaciones de Eucalyptus cladocalyx establecidos en la Región de Coquimbo. Se consideró al florecimiento como una respuesta binaria medida a los 30 meses de edad. Por lo tanto, se ajustó un modelo linear generalizado para analizar el efecto de procedencias. Posteriormente, se estudió la variabilidad genética dentro de la mejor procedencia, a través de inferencia Bayesiana, vía algoritmo de Gibbs. Inicialmente, se consideraron dos sitios, Hacienda Caracas y Tunga Norte, sector costero e interior de la Provincia de Choapa, respectivamente. En el sector costero, sólo un 1,33% de los árboles presentaron eventos de florecimiento, mientras que en Tunga Norte, este valor fue de 23,43%. Debido a este...
Tipo: Journal article Palavras-chave: Ambientes áridos; Árboles multipropósito; Eventos de florecimiento; Mejoramiento; Procedencias.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202007000200006
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Effects of insect cadavers infected by Heterorhabditis bacteriophora and Steinernema diaprepesi on Meloidogyne incognita parasitism in pepper and summer squash plants Ciencia e Investigación Agraria
Del Valle,Eleodoro E; Lax,Paola; Rondán Dueñas,Juan; Doucet,Marcelo E.
The effects of insect cadavers infected with three isolates of Heterorhabditis bacteriophora and one isolate of Steinernema diaprepesi on a population of Meloidogyne incognita in pepper (Capsicum annuum) and summer squash (Cucurbita maxima) were evaluated in greenhouse experiments carried out in Santa Fe (Argentina). Insect cadavers were obtained for the experiments from last instar larvae of Galleria mellonella and Tenebrio molitor that had been infected with entomopathogenic nematodes. Two six-day-old insect cadavers per pot were placed below the soil surface, and the soil was inoculated with 100 second-stage juveniles ofM incognita. Sixty days after inoculation, the following parameters were recorded for each plant: number of leaves; dry weight of...
Tipo: Journal article Palavras-chave: Biological control; Entomopathogenic nematodes; Insect host cadavers.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100009
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Mapeo de loci de caracteres cuantitativos (QTL) usando un enfoque multivariado Ciencia e Investigación Agraria
Mora,Freddy; Santos,Alexandra I; Scapim,Carlos A.
Los procedimientos usados en el mapeo de loci de característica cuantitativa (QTL) son continuamente estudiados ya que son fundamentales para mejorar la precisión del análisis genético. El presente estudio tuvo como objetivo examinar QTLs a través de métodos muí ti variados, considerando el principio de mapeo por intervalo simple. Se simuló un conjunto de datos de marcadores moleculares microsatélites provenientes de una población F2. Al mismo tiempo se consideró que los QTLs controlan características de distribución normal y binomial. En el caso normal se consideraron cinco modelos con las siguientes estructuras de covarianzas residuales: Componentes de varianza (VC), simetría compuesta (CS), no estructurada (UN), diagonal principal (Banded Main Diagonal;...
Tipo: Journal article Palavras-chave: Análisis multivariado; Binario; Ecuaciones generalizadas; Marcador molecular; Mejoramiento genético; QTL.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202008000200003
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Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile Ciencia e Investigación Agraria
Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S...
Tipo: Journal article Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018
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Colonization of blueberry (Vaccinium corymbosum) plantlets by ericoid mycorrhizae under nursery conditions Ciencia e Investigación Agraria
Carrillo,Rubén; Guerrero,Jaime; Rodríguez,Mario; Meriño-Gergichevich,Cristian.
R. Carrillo, J. Guerrero, M. Rodríguez, and C. Meriño-Gergichevich. 2015. Colonization of blueberry (Vaccinium corymbosum L.) plantlets by ericoid mycorrhizae under nursery conditions. Cien. Inv. Agr. 42(3): 365-374. The ericoid mycorrhiza is a symbiotic interaction that contributes to the improvement of the establishment and production of blueberries (Vaccinium corymbosum L.). The objectives of this paper were to assess the frequency (%) and intensity (%) of the colonization and growth of blueberry plantlets inoculated with ericoid mycorrhizae collected from three different edaphoclimatic conditions in the La Araucanía region of southern Chile under nursery conditions. Plantlets of three blueberry cultivars ("Brigitta", "Duke" and "Legacy") were grown...
Tipo: Journal article Palavras-chave: Blueberry; Ericoid mycorrhizae; Micorrhizal colonization.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000300005
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Variables explicativas de los flujos de compra de alimentos en las Regiones VIII, IX y X de Chile Ciencia e Investigación Agraria
Schnettler,Berta L; Rebollo,Alfonso.
La desigual distribución del equipamiento comercial provoca desplazamientos de compra desde los centros de menor tamaño hacia los de mayor dotación comercial, provocando evasiones de gasto comercializable o flujos comerciales. Para determinar el efecto de variables de atracción y de restricción en los flujos comerciales de alimentos en las regiones VIII, IX y X de Chile, se estimó el modelo de gravitación tradicional. Los atributos de los centros de compra fueron población, ingreso monetario mensual de los hogares, cantidad de patentes y la superficie comercial de establecimientos de venta de alimentos. Como variable de restricción se utilizó la distancia y el tiempo de desplazamiento. Los consumidores que realizan desplazamientos de compra fuera de la...
Tipo: Journal article Palavras-chave: Áreas comerciales; Desplazamientos de compra; Gravitación comercial.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202007000200002
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Cold storage effects on oxidative stress of Red Globe table grape rachises Ciencia e Investigación Agraria
Campos-Vargas,Reinaldo; Zamora,Pablo; Contreras,Rodrigo; Köhler,Hans; Zúñiga,Gustavo E; Pérez-Donoso,Alonso; Defilippi,Bruno G.
Table grape (Vitis vinifera L.) quality includes the condition of both the berries and the rachis. In the present report, physiological parameters of Red Globe rachises from fully elongated inflorescences (RFEI) and from mature clusters were studied after storage at 0 or 20 °C for different durations. To understand changes in rachis physiology as a result of changes in temperature conditions and storage time, the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were measured. In addition, hydrogen peroxide content, membrane lipoperoxidation (TBARS), total phenolic compounds and antioxidant capacity (FRAP) were assayed. TBARS was higher in mature rachises than in RFEI. This parameter remained constant throughout...
Tipo: Journal article Palavras-chave: Fruit quality; Rachis; Storage; Table grape; Vitis vinifera L.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100007
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Wind-induced leaning (toppling) in young Pinus radiata plantations in Chile Ciencia e Investigación Agraria
Toral,Manuel; Bown,Horacio E; Mañon,Alejandro; Alvarez,José; Navarro-Cerrillo,Rafael.
The toppling of young trees considerably reduces the value of the first log in Pinus radiata plantations. Although wind is the main cause of toppling, the quality and type of plants, planting techniques, soil fertility and soil preparation may play an important role in the susceptibility of the species to toppling. This article analyzes the influence of ripping and soil type on the probability of P radiata toppling after severe windstorms in central south Chile. To this aim, pairs of similar trees with and without toppling were selected in 2001 in six soil types with and without soil ripping. Root system differences in toppled versus straight trees were compared to gain better insight into the causes of the phenomenon. Among all root measurements, the...
Tipo: Journal article Palavras-chave: Ripping; Soil fertility; Tap root; Toppling.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000300010
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Unveiling Peruvian organic consumers demand for organics: A latent class approach Ciencia e Investigación Agraria
Higuchi,Angie; Dávalos,Jorge.
A. Higuchi, and J. Dávalos. 2016. Unveiling Peruvian organic consumers demand for organics: A latent class approach. Cien. Inv. Agr. 43(3): 408-417. This paper seeks to identify the consumer segments that constitute the market for organic products in Metropolitan Lima, Peru. The employed classification methodology, Latent Class Analysis, is implemented within a structural framework that is capable of identifying key relations such as the link between educational attainment and organic product consumption. This study builds on primary data gathered at the study site involving interviews with 547 organic consumers at a limited number of bio-fairs and bio-shops. Our results identify two segments: core and middle-level consumers. Although both segments exhibit...
Tipo: Journal article Palavras-chave: Attributes; Latent class analysis; Metropolitan Lima; Organic consumers; Peru; Socio-economic characteristics.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300007
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Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Ciencia e Investigación Agraria
Bastías,Adriana; Almada,Rubén; Rojas,Pamela; Donoso,José Manuel; Hinrichsen,Patricio; Sagredo,Boris.
A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172...
Tipo: Journal article Palavras-chave: Development; In vitro culture; Hase change; Rejuvenation.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009
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Influence of long-chain fatty acids on weight gain of Hylastinus obscurus (Coleoptera: Curculionidae) Ciencia e Investigación Agraria
Toledo,Dayand; Parra,Leonardo; Mutis,Ana; Ortega,Fernando; Hormazábal,Emilio; Quiroz,Andrés.
The contents of long-chain fatty acids in non-polar root extracts from 1.6 year-old red clover plants of two cultivars (Superqueli INIA and Quiñequeli INIA) and one experimental line (Syn II Pre III), their relationships with H. obscurus populations, and the stimulant or deterrent effects of the root extracts and of individual fatty acids on the weight gain of H. obscurus were investigated. There was an inverse relationship between the insect population size and the palmitic acid content. The Superqueli INIA cultivar contained the highest palmitic acid content and the lowest insect load. When solutions of either palmitic or oleic acids were individually incorporated into an artificial diet, palmitic acid caused a decrease in weight gain, whereas the effect...
Tipo: Journal article Palavras-chave: Deterrent; Hylastinus obscurus; Trifolium pratense; Palmitic acid.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000300008
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Production systems, technical parameters and quality of bovine milk producers in southern Chile Ciencia e Investigación Agraria
Pérez,Jorge A.
Production information of 29 milk producers in the southern region of Chile was studied with the objective of characterizing and classifying different dairy production systems, evaluating various associations between quantitative and qualitative studied variables and analyzing the effect of the production system and the quality of bovine milk according to the month of the year that it was processed. The classification of production groups was based on the implementation of the exploratory multivariate technique, main components analysis and conglomerates or cluster analysis. The applied statistical model was: y ijk = µ + Mi + SPj + MSPij + e ijk, where y ijk=dependent variables (fat, protein, count of somatic cells and colony forming units); µ=general...
Tipo: Journal article Palavras-chave: Cluster analysis; Colony forming units; Fat percentage; Main components analysis; Multivariable analysis; Protein percentage; Somatic cell.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100002
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Inbreeding depression and genetic components for popping expansion and other traits in Brazilian populations of popcorn Ciencia e Investigación Agraria
Arnhold,Emmanuel; Soriano Viana,José Marcelo; Mora,Freddy; Vieira Miranda,Glauco; Gonçalves Silva,Ricardo.
The aim of the study was to assess inbreeding depression and determine genetic components in landrace populations of popcorn. Six Brazilian popcorn landraces: Amarelo, Beija-Flor, Branco, Rosa-claro, Roxo and Viçosa, were subjected to self-pollination. Experiments with SO populations and their selfing populations (SI) were conducted in a randomized complete block design. The estimates of inbreeding depression varied from 1.2% (popping expansion) to 79% (grain yield). The gene action was predominantly additive for plant height, ear height and hundred-grain-weight. The dominance genetic effects were important for index of prolificacy and grain yield. The genetic effect of popping expansion was bidirectional with nuil dominance and a predominance of additive...
Tipo: Journal article Palavras-chave: Zea mays L; Mprovement; Popping expansion; Popcorn; Genetic variance.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300011
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Participation determinants and impact assessment of the Institute of Agricultural Development (INDAP) credit program Ciencia e Investigación Agraria
Donoso,Guillermo; Melo,Oscar; Negrete,Eduardo.
This research estimates the impact of INDAP's Credit Program on the per hectare agricultural income of small agricultural producers. To accomplish this, a Treatment Effects Model (TEM) is employed to simultaneously estímate producer incomes and program participation functions, correcting for the potential presence of selectivity bias through the correlation between the errors of both functions. The results indicate that farmers of greater age present a higher disposition to participate in the program, but their interest decreases as their age increases. Additionally, producers who are acquainted with and evaluate the financial cost of credits as well as those producers whose income comes mainly from their land, who are landowners, present a greater family...
Tipo: Journal article Palavras-chave: Program impact estimation; Selection bias; Rural finance; Treatment effects model.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000200009
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Presence of tetracycline resistant bacteria and genes in grassland-based animal production systems Ciencia e Investigación Agraria
López,Liliana; Santamaría,Johanna; Sánchez,Alexandra; Castro,Lida; Moreno,Jessica L.
This study assessed the presence of tetracycline-resistant bacteria in five different grassland-based production systems dedicated to raising dairy cattle located in the Colombian Andes. Animal (ruminal fluid and feces) and environmental (soil and water) samples were evaluated, and resistant heterotrophic bacteria were found in rumen fluid, feces, soil, and runoff water samples. The resistant bacteria were isolated and identified based on the 16S rDNA region. Subsequently, they were evaluated for the presence of tet genes, which encode ribosomal protection proteins and membrane efflux pumps. The most frequent phyla detected were Firmicutes and Proteobacteria. The most common resistance genes found were tet(W), tet(Q), and tet(M). The nucleotide sequences...
Tipo: Journal article Palavras-chave: Antimicrobial resistance; Cattle farms; Colombian Andes; Environmental pollution; Tetracycline.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300002
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Variation over time in the prevalences of three viruses of the grapevine leafroll complex in a commercial vineyard in south-central Chile Ciencia e Investigación Agraria
Mujica,María Valentina; Mora,Roxana; Rosales,Marlene; Sandoval,Claudio.
A vineyard in south-central Chile was surveyed during five growing seasons to analyze the increase over time in the number of plants infected with Grapevine leafroll-associated virus-1, -2 and -3. At the end of the study, GLRaV-3 was found to be the most prevalent virus, with 46% of plants infected by this pathogen. GLRaV-2 infected 13% of plants, and GLRaV-1 infected 12%. Considering all three viruses together, the prevalence of infection increased exponentially from 14% of the plants at the beginning of the study period to 68% at the end. The patterns of the spread of GLRaV-2 and GLRaV-3 were mostly along the rows, whereas the pattern of spread for GLRaV-1 could not be determined.
Tipo: Journal article Palavras-chave: Field transmission; Grapevine leafroll-associated virus; GLRaV-1; GLRaV-2; GLRaV-3; Virus diagnosis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100012
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Relative susceptibility of peach rootstocks to crown gall and Phytophthora root and crown rot in Chile Ciencia e Investigación Agraria
Guzman,Gonzalo; Latorre,Bernardo A; Torres,Rene; Wilcox,Wayne F.
Crown gall (Agrobacterium tumefaciens) and Phytophthora root and crown rot (PRCR), caused by P. cryptogea, are two major limiting factors affecting peach (Prunus pérsica) production in Chile. At present, Nemaguard is the most important rootstock used in peach orchards, and although several clonal rootstocks are under development worldwide, there is little information regarding their susceptibility to crown gall and PRCR. Therefore this investigation was undertaken to study the susceptibility to A. tumefaciens and P. cryptogea of the clonally propagated rootstocks Cadman®-Avimag (P. pérsica x P. davidiana), Mr.S 2/5 (P. cerasifera x P. spinosa), Viking ([P. pérsica x P. davidiana] x [P. dulcís 'Jordanolo'x P. blireiana]), and Nemaguard (P. pérsica x P....
Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; Disease control; Nectarines; Rootstock resistance.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202007000100004
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Stability of anthocyanin in spinach vine (Basella rubra) fruits Ciencia e Investigación Agraria
Ferreira Ozela,Eliana; Stringheta,Paulo César; Cano Chauca,Milton.
The stability of anthocyanin in the extract of spinach vine fruit (Basella rubra L.) was studied in relation to degradative factors such as light, temperature and pH acting alone or in combination. In this work, the possible use of spinach vine fruit as a source of natural pigments for use in food coloring was evaluated. Extraction of the pigment was carried out with 99.9% methanol at pH 2.0. The stability of the anthocyanin extract was estimated. From these values, reaction velocity constants (k) as well as the half-life time (t l;2) were calculated at pH 4.0, 5.0, and 6.0 in the presence and absence of light both at 40 and 60°C. Results indicate that, independent of pH values, spinach vine extract suffered an interference of light in its anthocyanin...
Tipo: Journal article Palavras-chave: Anthocyanin; Bertalha; Coloring; Food color; Spinach vine.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202007000200004
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