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| Registros recuperados: 240 | |
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| Ocarino,N.M.; Bozzi,A.; Pereira,R.D.O.; Breyner,N.M.; Silva,V.L.; Castanheira,P.; Goes,A.M.; Serakides,R.. |
| 4&', 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them to neighboring cells, while DAPI-treated cells were mostly round cells with fine and short extensions.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bone marrow; Cell cultures; Rat; Osteogenic differentiation. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200003 |
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| Villalobos,Virginia; Bonilla,Ernesto; Castellano,Alan; Novo,Ernesto; Caspersen,Ralph; Giraldoth,Debora; Medina-Leendertz,Shirley. |
| The effect of manganese toxicity on the ultrastructure of the olfactory bulb was evaluated. Male albino mice were injected intraperitoneally with MnCl2 (5 mg/Kg/day) five days per week during nine weeks. The control group received NaCl (0.9%). The olfactory bulbs of five mice from each group were processed for transmission electron microscopy after 2, 4, 6 and 9 weeks of manganese treatment. On week 2, some disorganization of the myelin sheaths was observed. After 4 weeks, degenerated neurons with dilated cisternae of rough endoplasmic reticulum and swollen mitochondria appeared. A certain degree of gliosis with a predominance of astrocytes with swollen mitochondria, disorganization of the endomembrane system, dilation of the perinuclear cisternae and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metal toxicity; Toxicity; Neurodegeneration; Electron microscopy. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300007 |
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| Caro,Luis A.; Santecchia,Natalia; Marinangeli,Pablo A.; Curvetto,Néstor R.; Hernández,Luis F.. |
| The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium rhizogenes; Algarrobo; Hairy roots; Indole-3-butyric acid; Nothofagus nervosa; Prosopis chilensis; Raulí; Rooting. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300002 |
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| Barrios,Lilian; Poletti,Oscar Héctor. |
| We have evaluated the effect of filgrastim on proliferation and differentiation activity of granulopoietic cells in mice pretreated with methotrexate. Filgrastim was injected daily, from day 8 to 28 after cytotoxic agent administration. The granulopoiesis changes were measured by assessment of GM-CFU cells content, marrow and spleen granuloid cells pool as well as circulating neutrophils. In MTX pretreated mice, bone marrow GM-CFU oscillating values were higher than normal values, but these changes were not followed by high proliferative activity in granuloid precursor cell compartment. After MTX treatment, filgrastim administration was unable to stimulate marrow granulopoiesis as observed in normal mice. In the spleen, MTX led to dramatic changes in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Methotrexate; Filgrastim; Granulopoiesis; Bone marrow; Spleen. |
| Ano: 2005 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100002 |
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| Kohli,Alicia; Pezzotto,Stella M.; Garcia,Graciela; Poletto,Leonor C.. |
| The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin, periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results with the erosion technique. Thirty human teeth were employed in the trial; after their extraction the pulp was fixated, the pieces... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulp dentine organ; Odontoblast; Odontoblast process. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000200003 |
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| Olivella,Mónica S.; Debattista,Nora B.; Pappano,Nora B.. |
| Considering the skin´s function, different dermal pharmaceutical forms can be developed according to the type of therapeutic activity, active principle and excipients involved in the formulation, such as transdermal preparations. In the present study, the permeation parameters of the non-steroidal antiinflammatory drug, salicylic acid (SA) through synthetic membrane, polyvinyliden difluoride, and a biological membrane, egg shell membrane, with different vehicles, propylene glycol, isopropyl alcohol and carbopol gel, were determined. The reported physicochemical parameters of SA from CG were significantly higher than those obtained using PG and IP. This is attributed to the lipophilic nature of the vehicle that facilitates the release and penetration of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Membranes; Permeation; Drug release; Salicylic acid. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200008 |
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| Mardegan Issa,João Paulo; Tiossi,Rodrigo; Mizusaki Iyomasa,Mamie. |
| The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals' perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and T groups in width (p<0.05). Microscopically, C and T groups showed polygonal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Masseter muscle; Morphology; Histology; Histochemistry. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300003 |
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| Vigliocco,A.; Bonamico,B.; Alemano,S.; Miersch,O.; Abdala,G.. |
| In the present paper we study the possible biological relevance of endogenous jasmonic acid (JA) and exogenous salicylic acid (SA) in a plant-microbial system maize-virus. The virus disease "Mal de Río Cuarto" is caused by the maize rough dwarf virus - Río Cuarto. The characteristic symptoms are the appearance of galls or "enations" in leaves, shortening of the stem internodes, poor radical system and general stunting. Changes in JA and protein pattern in maize control and infected plants of a virus-tolerant cultivar were investi-gated. Healthy and infected-leaf discs were collected for JA measurement at different post-infection times (20, 40, 60 and 68 days). JA was also measured in roots on day 60 after infection. For SDS-PAGE protein analysis, leaf... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Maize; Virus disease; Jasmonic and salicylic acids. |
| Ano: 2002 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300007 |
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| Registros recuperados: 240 | |
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