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Age-age correlation for early selection of rubber tree genotypes in São Paulo State, Brazil Genet. Mol. Biol.
Gonçalves,Paulo de Souza; Bortoletto,Nelson; Cardinal,Átila Bento Beleti; Gouvêa,Lígia Regina Lima; Costa,Reginaldo Brito da; Moraes,Mário Luiz Teixeira de.
In a field trial involving 68 rubber tree (Hevea spp.) clones calculation of genotypic correlation coefficients revealed significant age-age correlation from age 1 to 6 (immature period) for girth A and for age 7 to 12 (mature period) for girth B and for age 7 to 12 (production of latex) for yield. Rank correlation coefficients between all immature ages of girth (girth A), all ages of mature girth (girth B) and all annual rubber production (yield) were significant for the three traits, with the coefficients decreasing with increasing age. Selection of the sets of best 30, 15, 10 and 5 clones from the available 68 clones at a given age was generally accompanied by a descending order of percentage success. It was suggested: (a) to have the best 30 clones of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hevea brasiliensis; Early clonal multiplication; Genetic correlation; Rank correlation; Evaluation of clones.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500018
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Aggregation tendency in small groups of Drosophila melanogaster Genet. Mol. Biol.
del Solar Osses,Eduardo.
A study was made of the spatial pattern of the progeny produced in boxes with 25 oviposition tubes, by groups of six Drosophila melanogaster females during 24, 72 and 216 h. Each female was fertilized by a male carrier of a dominant mutation in such a manner that the behavior of each female of the group as well as that of each group could be determined by a study of their progeny. The pattern of spatial distribution of the progeny was aggregated, reaching its highest value at 72 h. Gregarious behavior of individuals was defined by the number of sites among which they disseminated their progeny in a 24 h-cycle; while for groups it was estimated by the variance/mean ratio. The fertility, egg-adult development time and mortality rate were recorded....
Tipo: Info:eu-repo/semantics/article
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100006
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Agmatoploidy and symploidy: a critical review Genet. Mol. Biol.
Guerra,Marcelo.
Abstract Agmatoploidy is a type of chromosome rearrangement that involves the fragmentation of an entire chromosome complement, generating a diploid with double its original chromosome number. Agmatoploidy and other related karyotype changes, such as symploidy (the opposite change, promoted by chromosome fusion), partial agmatoploidy, polyagmatoploidy, etc., are restricted to species with holokinetic chromosomes and are assumed to play an important role in their karyotype evolution. However, a critical review of the literature shows that examples of chromosome number doubling by agmatoploidy are rare and not clearly demonstrated, while partial agmatoploidy and partial symploidy seem to be the same as ascending and descending disploidy, respectively. It is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agmatoploidy; Symploidy; Holocentrics; Holokinetic chromosomes; Chromosome evolution.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400492
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Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency Genet. Mol. Biol.
Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D..
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022
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Agronomic, cytogenetic, and isoenzymatic characterizations of oat somaclones Genet. Mol. Biol.
Augustin,Lizete; Federizzi,Luiz Carlos; Sereno,Maria Jane Cruz de Melo.
Immature embryo-derived somaclones regenerated from genotypes UPF 12, UPF 89S080 and UFRGS 7 were analyzed for eight agronomic traits and two enzymatic systems in order to evaluate the potential of tissue cultures to induce genetic variability in oats (Avena sativa L.). Some somaclones were also analyzed cytogenetically. Agronomic traits were evaluated in the field for all somaclones in 1993 and 1994. Bi-directional variation (P < 0.05) was detected for all characteristics, and the average frequency of variation observed in 1993 in somaclones from genotypes UPF 12 and UPF 89S080 was 35.2%. Variation observed for agronomic characteristics was heritable through two generations. Isoenzymatic analysis showed variation for both enzymatic systems in four...
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300025
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Alcohol dehydrogenase activities and ethanol tolerance in Anastrepha (Diptera, Tephritidae) fruit-fly species and their hybrids Genet. Mol. Biol.
Carvalho,Eneas; Solferini,Vera Nisaka; Matioli,Sergio Russo.
The ADH (alcohol dehydrogenase) system is one of the earliest known models of molecular evolution, and is still the most studied in Drosophila. Herein, we studied this model in the genus Anastrepha (Diptera, Tephritidae). Due to the remarkable advantages it presents, it is possible to cross species with different Adh genotypes and with different phenotype traits related to ethanol tolerance. The two species studied here each have a different number of Adh gene copies, whereby crosses generate polymorphisms in gene number and in composition of the genetic background. We measured certain traits related to ethanol metabolism and tolerance. ADH specific enzyme activity presented gene by environment interactions, and the larval protein content showed an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alcohol dehydrogenase; Anastrepha; Hybrids; Ethanol tolerance; Tephritidae.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100027
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Alexandre Afranio Peixoto (1963-2013) Genet. Mol. Biol.
Klaczko,Louis Bernard; Bruno,Rafaela V..
Tipo: Info:eu-repo/semantics/other
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200001
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Allele frequency and selection efficiency in cross populations of Andean x Mesoamerican common beans (Phaseolus vulgaris L. Fabales, Fabaceae) Genet. Mol. Biol.
Baldoni,Aisy B.; Ramalho,Magno A. Patto; Abreu,Ângela de Fátima B..
Strategies were investigated for improving efficiency in the use of segregating common bean (Phaseolus vulgaris) populations using crosses between the Andean cultivar BRS-Radiante and the Mesoamerican parent cultivar Carioca-MG by developing populations with 12.5%, 25%, 50%, 75% and 87.5% of the allele frequency of one of the parents. For each of the five populations we evaluated for two traits, the number of days to the beginning of flowering and grain yield (g plot-1), in the F2:3 (sown in February 2006) and F2:4 (sown in July 2006) generation progenies using 15 x 15 lattice design experiments, with 44 progenies (n = 220 plants) plus the two parents and three controls being evaluated for each generation. In terms of variability release, the populations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Backcross; Gene pools; Heritability; Phaseolus vulgaris; Selection gain.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500019
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Allele frequency distributions of six hypervariable loci (D1S80, APOB, D4S43, vW1, F13A and DYS19) in two African-Brazilian communities from the Amazon region Genet. Mol. Biol.
Vallinoto,Izaura Maria Vieira Cayres; Vallinoto,Antonio Carlos Rosário; Valente,Cristina Maria Duarte; Guerreiro,João Farias.
The allele frequency distributions of three VNTR (D1S80, APOB and D4S43) and three STR (vW1, F13A1 and DYS19) loci were investigated in two Afro-Brazilian populations from the Amazon: Curiau and Pacoval. Exact tests for population differentiation revealed significant differences in allele frequency between populations only for the D1S80 and APOB loci. A statistically significant deviation from the Hardy-Weinberg equilibrium was observed only in the D1S80 locus of the Pacoval sample. A neighbor-joining tree was constructed based on DA genetic distances of allele frequencies in four Afro-Brazilian populations from the Amazon (Pacoval, Curiau, Trombetas, and Cametá), along with those from Congo, Cameroon, Brazilian Amerindians, and Europeans. This analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA polymorphisms; Afro-Brazilians; Hypervariable loci.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300003
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Allelic frequencies and statistical data obtained from 12 codis STR loci in an admixed population of the Brazilian Amazon Genet. Mol. Biol.
Francez,Pablo Abdon da Costa; Rodrigues,Elzemar Martins Ribeiro; Frazão,Gleycianne Furtado; Borges,Nathalia Danielly dos Reis; Santos,Sidney Emanuel Batista dos.
The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (F ST coefficients) to the present database ranged from F ST = 0.0016 between Macapá and Belém to F ST =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelic frequencies; Brazilian Amazon population; Interethnic admixture.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100007
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Allelic relationships of anthracnose (Colletotrichum lindemuthianum) resistance in the common bean (Phaseolus vulgaris L.) cultivar Michelite and the proposal of a new anthracnose resistance gene, Co-11 Genet. Mol. Biol.
Gonçalves-Vidigal,Maria Celeste; Silva,Claudete Rosa da; Vidigal Filho,Pedro Soares; Gonela,Adriana; Kvitschal,Marcus Vinícius.
The genetic resistance of Phaseolus vulgaris L. cultivar Michelite to races 8 and 64 of Colletotrichum lindemuthianum, causal agent of bean anthracnose, was characterized. Crosses were made between Michelite and Mexico 222 cultivars and the F2 population was inoculated with race 64 in order to study the inheritance of resistance to anthracnose in Michelite. The segregation of F2 population fitted in a ratio of 3R:1S, showing the presence of a dominant gene in Michelite gene conditioning resistance to race 64. Allelism tests were conducted with F2 populations derived from crosses between Michelite and AB 136, AND 277, BAT 93, Cornell 49-242, G 2333, Kaboon, Mexico 222, Michigan Dark Red Kidney (MRDK), Ouro Negro, Perry Marrow, PI 207262, TO, TU, and Widusa....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colletotrichum lindemuthianum; Gene pool; Mesoamerican resistance gene; Phaseolus vulgaris L..
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400015
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Allozyme comparison of two populations of Rineloricaria (Siluriformes, Loricariidae) from the Ivaí River, upper Paraná River basin, Brazil Genet. Mol. Biol.
Limeira,Daniel M.; Renesto,Erasmo; Zawadzki,Cláudio H..
Two allopatric morphotypes of the genus Rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, R. pentamaculata, from the Keller River in the middle stretch of the Ivaí River basin and the other, R. aff. pentamaculata, from the São João River in the upper portion of the Ivaí River basin. The morphotype from the São João River was collected upstream from the São João waterfall, which is about 80 m deep. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, which allowed to score 22 loci. Only loci Aat-2, Est-3 and Mdh-C showed polymorphism. The two samples differed in allele frequencies at the three polymorphic loci. The average expected heterozygosity for all loci was...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Allozymes; Fish genetics; Genetic distance; Polymorphism; Loricariidae; Pisces.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200034
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Allozyme differentiation of two populations of the genus Neoplecostomus Eigenmann &amp; Eigenmann, 1888 (Teleostei, Loricariidae) from the upper Paraná River basin, Brazil Genet. Mol. Biol.
Reusing,Ana Flávia; Renesto,Erasmo; Roxo,Fábio F.; Zawadzki,Cláudio H..
Allozyme electrophoresis was used to examine 12 enzymatic systems in two populations of the genus Neoplecostomus from the Paraná River basin. Samples of Neoplecostomus sp. 1 were collected in Paraitinguinha stream of the Tietê River basin, in the municipality of Salesópolis, São Paulo State, and those of Neoplecostomus sp. 2 from São Domingos stream of the Rio Grande River basin, in the municipality of Muzambinho, Minas Gerais State. The genetic variability of the two populations was estimated by Nei's expected heterozygosity and was considered lower than average for populations of freshwater fish. The proportion of polymorphic loci was low (only 5.26% for the locus Idh). The low frequency of heterozygosity for both populations revealed a high fixation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Genetic variability; Neoplecostominae; Neotropical fishes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300021
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Allozyme variation among three populations of the armored catfish Hypostomus regani (Ihering, 1905) (Siluriformes, Loricariidae) from the Paraná and Paraguay river basins, Brazil Genet. Mol. Biol.
Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana.
Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025
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Allozyme variation and sociogenetic structure of Polistes satan Bequaert 1940 colonies (Hymenoptera, Vespidae) Genet. Mol. Biol.
Gaspar,Jane Cristina Wehren; López-Uribe,Margarita María; Del Lama,Marco Antônio.
We collected 40 colonies of the wasp Polistes satan in November 1993 (Population A = 23 colonies) and October 1995 (Population B = 17 colonies) from the town of Delfinopólis in the Brazilian state of Minas Gerais. Pupae from these nests were used to assess allozyme variation and genetic relatedness among nestmates. Of the 24 gene loci sampled six showed allozyme polymorphism (Est-2, Pgm-1, 6Pgd, Hk-1, Idh and Pep-A) and all loci except Idh were at Hardy-Weinberg equilibrium. Populations A and B showed distinct intra-loci heterozygosities, but similar average heterozygosity over all loci. Significant inter-population differentiation (Fst = 0.051; p < 0.01) was detected, probably due to genetic drift driven by bottlenecking that occurred due to the first...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Gene variation; Polistes satan; Relatedness; Wasps.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300028
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Allozymic variation and differentiation in the chilean blue mussel, Mytilus chilensis, along its natural distribution Genet. Mol. Biol.
Toro,Jorge E.; Castro,Grace C.; Ojeda,Johana A.; Vergara,Ana M..
Genetic differentiation in the Chilean blue mussel Mytilus chilensis (Hupé 1854) was investigated based on the variation in the allozyme frequencies of Pgm, Gpi, Icd, Me, Gsr, Lap and Pep in eight samples collected along 1800 km from Arauco (VIII Region) to Punta Arenas (XII Region). Despite the large geographic separations, values of Neis unbiased genetic distance, D (0.004-0.048) and standardised genetic variation among populations, Fst (0.011-0.055) were small. The levels of gene flow (Nm = 8) found in this study prevent the effect of differentiation among populations by genetic drift. This findings indicate that its long-lived planktotrophic larvae provides this species with considerable dispersal ability throughout its range which is favoured by the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozyme; Mytilus chilensis; Population genetics; Gene flow; Chile.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100031
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Alpha-1 antitrypsin gene polymorphism in Chronic Obstructive Pulmonary Disease (COPD) Genet. Mol. Biol.
Denden,Sabri; Khelil,Amel Haj; Knani,Jalel; Lakhdar,Ramzi; Perrin,Pascale; Lefranc,Gérard; Chibani,Jemni Ben.
Alpha-1-antitrypsin (AAT) plays an important role in the pathogenesis of emphysema, the pathological lesion underlying the majority of the manifestations of Chronic Obstructive Pulmonary Disease (COPD). In this study we tested the hypothesis that common AAT polymorphisms influence the risk of developing COPDs. We investigated PiM1 (Ala213Val), PiM2 (Arg101His), PiM3 (Glu376Asp), PiS (Glu264Val) and PiZ (Glu342Lys) SERPINA1 alleles in 100 COPD patients and 200 healthy controls. No significant differences were observed in allele frequencies between COPD patients and controls, neither did haplotype analysis show significant differences between the two groups. A cross-sectional study revealed no significant relationship between common SERPINA1 polymorphisms...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha-1 antitrypsin; SERPINA1 polymorphisms; COPD; Emphysema; Lung function.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100006
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alpha-thalassemia, HbS, and beta-globin gene cluster haplotypes in two Afro-Uruguayan sub-populations from northern and southern Uruguay Genet. Mol. Biol.
Luz,Julio A. da; Sans,Mónica; Kimura,Elza Miyuki; Albuquerque,Dulcinéia Martins; Sonati,Maria de Fatima; Costa,Fernando Ferreira.
Hemoglobinopathies are the most common monogenic disorders worldwide; however, they have never been systematically studied from a genetic perspective in Uruguay. In this study, we determined the frequencies of hemoglobin variants in Afro-Uruguayans. A sample of 52 healthy unrelated Afro-Uruguayans from the northern (N = 28) and southern (N = 24) regions of the country was analyzed. Eight individuals (15.4%) were heterozygous for -alpha3,7thalassemia; seven of them (29.2%) were originally from the southern region, whereas one of them (3.6%) was from the northern region; the differences between both regions were statistically significant (p = 0.016 +/-0.003). The only structural mutation detected was betaS, which is typical of African populations. Four...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemoglobinopathies; Alpha-thalassemias; HbS; Haplotypes; Afro-derived populations.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400002
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Alterations in DNA repair gene expression and their possible regulation in rat-liver regeneration Genet. Mol. Biol.
Wang,Gai-Ping; Xu,Cun-Shuan.
Rapidly proliferating tissue may require enhanced DNA repair capacity in order to avoid fixation of promutagenic DNA lesions to mutations. Partial hepatectomy (PH) triggers cell proliferation during liver regeneration (LR). However, little is known on how DNA repair genes change and how they are regulated at the transcriptional level during LR. In the present study, the Rat Genome 230 2.0 array was used to detect the expression profiles of DNA repair genes during LR, and differential expression of selected genes was confirmed by real-time RT-PCR. 69 DNA repair genes were found to be associated with LR, more than half of which distributed in a cluster characterized by a gradual increase at 24-72h and then returning to normal. The expression of base excision...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partial hepatectomy; Rat genome array; DNA repair genes; Liver regeneration.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200023
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Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line Genet. Mol. Biol.
Carminati,Patricia Oliveira; Mello,Stephano Spano; Fachin,Ana Lucia; Junta,Cristina Moraes; Sandrin-Garcia,Paula; Carlotti,Carlos Gilberto; Donadi,Eduardo Antonio; Passos,Geraldo Aleixo Silva; Sakamoto-Hojo,Elza Tiemi.
Gliomas are the most common tumors in the central nervous system, the average survival time of patients with glioblastoma multiforme being about 1 year from diagnosis, in spite of harsh therapy. Aiming to study the transcriptional profiles displayed by glioma cells undergoing cisplatin treatment, gene expression analysis was performed by the cDNA microarray method. Cell survival and apoptosis induction following treatment were also evaluated. Drug concentrations of 12.5 to 300 μM caused a pronounced reduction in cell survival rates five days after treatment, whereas concentrations higher than 25 μM were effective in reducing the survival rates to ~1%. However, the maximum apoptosis frequency was 20.4% for 25 μM cisplatin in cells analyzed at 72 h,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Cisplatin; Gene expression; Glioma.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100027
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